• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2019, Vol. 21 ›› Issue (5): 491-492.DOI: 10.3969/j.issn.1671-2587.2019.05.013

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Analysis of Band Patterns of Western Blot Test in 54 HIV Antibody Positive Blood Donors

CHEN Xiu-lian, HAN Xue-ying, LI Hua   

  1. Jinan Blood Supply and Security Center,Jinan 250001
  • Received:2018-07-03 Online:2019-10-20 Published:2019-10-29

Abstract: Objective To provide theoretical support for recruitment of blood donors,occupational protection of our staffers as well as blood test we did study on the band patterns of HIV antibody positive.Methods Whole blood samples of the blood donors’were tested by ELISA screening test in our laboratory and those HIV antibody reactive samples were sent to Ji Nan CDC for confirmatory test using Western blotting technology. Collect the positive information feedback and take a pooled analysis of band patterns by analyzing the rate of the band appearance as well as band patterns.Results Totally 54 positive cases were collected. Reactivity with all the bands was the most commonly observed WB pattern,which occurred 38.9%in HIV antibody positive blood donors. The bands of gp160/120 and p24 were observed in all the patients. The next is p66 (92.6%) and gp41(90.7%). The positive rate of p31,p17,p51 were 83.3%,87.0%,87.0% respectively. The bands of p39 and p55 showed low positive rates (59.2% and 42.6%,respectively).Conclusion The band patterns indicate that HIV infection is in the early stage in most of the HIV antibody positive blood donors. Measures should be taken to avoid blood donation from those high risk population.

Key words: HIV, Band pattern, Blood donors, Western blotting

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