Since the novel coronavirus (SARS-CoV-2) broke out in China at the end of 2019 and has been going around the world, it has become a global new public health threat. Scientists have had a preliminary understanding of the new coronavirus, and the research on the transmission route is getting deeper and deeper. It has been noted that SARS-CoV-2 has a long incubation period and a large number of asymptomatic infections, which is undoubtedly a major challenge to current blood safety. Is there any possibility that SARS-CoV-2 can be transmitted through blood transfusion? How do we ensure the safety of transfusion in clinical during epidemic prevention and reduce the risks of transmission of SARS-CoV-2 through blood transfusion? These are the major problems that the blood collection and supply agencies care about. As there has never been reported that respiratory borne virus can be transmitted through blood products, the risk that SARS-CoV-2 can be transmitted via blood transfusion is currently considered theoretically, based on the studies on acute respiratory syndrome (SARS) and middle East respiratory syndrome (MERS), both of which are caused by coronavirus infection as well. During the special period of time, the blood collection and supply institutions can take actions voluntarily to conduct intervention measures to minimize virus transmission through blood transfusion and eliminate people's panic on blood safety.

Objective To reengineer apheresis donation site and workflow during COVID-19 epidemic to ensure the safety of blood donor and staff. Methods Adjusting donation workplace settings, blood donation process, disinfection and cleaning, and staff safety protection, etc., to establish a safe, reliable and effective apheresis donation place and workflow that meets the requirements for epidemic prevention and control. Results By adjusting donation workplace settings and process reengineering, enhancing COVID-19 consultation and post-donation interview, and strengthening safety protection and disinfection and cleaning operations at work, not only 1397 platelet doses of 745 person-times were collected, but also no donors or staffs were infected with COVID-19 during COVID-19 epidemic. Conclusion During COVID-19 epidemic, it is necessary to reengineer apheresis donation site and workflow to meet the dual needs of epidemic prevention and collection of platelet, and provide a strong safety guarantee for the orderly apheresis donation work during COVID-19 epidemic.

Objective To establish a SARS-CoV-2 related detection platform suitable for pretransfusion laboratories to avoid biological risks. Methods An automated SARS-CoV-2 detection platform was established by modifying the automatic sampler program, adjusting the liquid curve, reducing the injection speed, performing sub-surface addition and mixing, editing the enzyme immunoassay and LIS program and optimizing the nucleic acid pooling program. Results The IgM and IgG antibody,IgG antibody with its titre,and nucleic acid of coronavirus in plasma from cured COVID-19 patients were successfully detected on the platform. Conclusion The established platform could realize automated and standardized sample addition and detection, and avoid biosafety risks. It may provide a favorable strategy for the recovery period plasma detection which could be helpful in COVID-19 treatment.

Objective To study the biosafety protection of blood transfusion departments (blood banks) in all types of hospitals during the epidemic situation of Novel coronavirus pneumonia. Methods Through the "Internet + WeChat Questionnaire Star" platform, blood transfusion departments (blood banks) of 2 309 hospitals in all provinces and cities were surveyed except Hong Kong, Macao and Taiwan. Results 1 876 in 2 309 blood transfusion departments (blood bank)of hospitals had the function of external ventilation and lighting windows (81.25%), 1042 (45.13%) had secondary biosafety laboratories, 597(25.86%)equipped with biosafety cabinets and and 224 (9.7%) equipped with automatic centrifuges. 1 498(64.88%) in 2309 blood transfusion departments (blood bank) use materials with osmotic function in laboratory operating table. The employment rate of employees reaching Grade I biosafety protection products is 88.7%~95.8%, while the utilization rate of reaching Grade II biosafety protection products is only 23.45% -56.08%. After the test, 2 039 (88.31%)blood samples were covered with test tube covers and 2 000 (86.32%)sealed with two yellow garbage bags, and 1 229 (53.23%) of blood samples were stored for 7 days. Conclusion Through this Internet + investigation, we should realise that the work of biological safety protection in the blood transfusion department (blood bank) has a long way to go.

Since the COVID-19 outbreak, the number of nationwide blood donors has plummeted, and the blood supply is facing a grim situation. In order to ensure blood supply emergency work during epidemic prevention and control, the national blood collection and supply agencies actively organize emergency group blood donation. Different from individual blood donation on the street, there are many people at the group blood donation sites, and the environment for blood donation is complex and diverse. Therefore, the prevention and control of group blood donation sites has become the primary task of blood collection and supply agencies during the COVID-19 the outbreak. Through practice, this article summarizes environmental cleaning and disinfection strategy, consultation and physical examination strategy, one-way mobile blood collection strategy, and strategy for prevention and treatment of donor adverse reactions for prevention and control of group blood donation sites during epidemic prevention and control for peer references.

Objective To compare ELISA and colloidal gold immunoassay reagents in IgG and IgM antibody titer detection in plasma from the COVID-19 convalescent patients (COVID-19 CP). To determine the effect of methylene blue/photochemical virus inactivation on the titer of 2019-nCoV IgG. Methods Blood samples were collected from 15 COVID-19 convalescent patients discharged from hospital in different time, and tested according to the blood station technical operation rules (2019 Edition). Novel coronavirus pneumonia (2019-nCoV) RNAs were detected by nucleic acid testing. 3 IgG and 2 IgM ELISA kits,1 colloidal gold reagent were used to detect the antibody of IgM and IgG respectively, after diluted by origin, 20, 40, 80, 160 and 320 times. The titers of IgG antibody were detected in 7 plasma samples before and after the virus inactivation by methylene blue to evaluate the effect of virus inactivation on the titer of antibody. Results 15 samples of COVID-19 CP were qualified, and the 2019-nCoV nucleic acid test was negative. The ALT levels were high. There was a significant difference in the titer of IgG detected by 3 IgG and 2 IgM ELISA reagent kits from different manufacturers. Conclusion Domestic reagents can detect the IgG and IgM of 2019-nCoV, but the titer levels are different from the different reagents. It is necessary to further study the methodology and sensitivity of qualitative reagent and gradient dilution method for the detection of antibody titer. Methylene blue/photochemical virus inactivation has no significant effect on the titer of 2019-nCoV IgG

Objective The study aimed to investigate the changes of autologous whole blood during the preservation in the third trimester of pregnancy and to provide theoretical guidance for preoperative autologous blood donationin pregnant women. Methods Twenty-eight late pregnancy women with autologous blood storage in our hospital were enrolled and divided equally into routine anticoagulant group (group 1) and high-anticoagulant group (group 2). At the sane time, 20 cases of conventional anticoagulated female unpaid blood were selected as control. Then we monitored the changes of blood coagulation function, blood viscosity, lactic acid concentration, blood potassium and blood appearance in 3 groups during preservation, and counted the utilization rate of blood in the obstetric group. Results The PT and APTT values of group 1 were significantly lower than control(P<0.05), and FIB concentration was significantly higher than control (P<0.01) at 1 h. During the preservation, compared with the control group, the APTT value and PT value of group 1 were gradually prolonged (P<0.05), EAI value, blood potassium and lactic acid concentration were gradually increased (P<0.05), FIB concentration were gradually decreased (P<0.05), but there was no significant difference between group 2 and the control group (P>0.05). The blood of group 1 showed fine particle agglutination on the 3rd day. There were 18, 11 and 10 bags of blood which showed Particle agglutination phenomenon; in group 1, group 2 and control group, respectively on the 35d.The utilization rate of preoperative autologous blood in obstetric group was 3.6% (1/28). Conclusion The blood of pregnant women in the third trimester is hypercoagulable. Conventional anticoagulated whole blood undergoes a change in blood quality during storage, manifested by increased lactic acid production, potassium concentration and blood viscosity, micro agglutination had occurred on the 3rd day. Increased proportion of anticoagulant during blood preservation can effectively solve this problem.

Objective To study the relationship between the blood type antibodies IgG subsets and the risk of newborns hyperbilirubinemia. Methods The serum of 50 cases high titer antibodies gravidaes and their newborns were carried out the serology detection（ABO 41 cases and Rh 9 cases）.The IgG subsets and serum bilirubin levels of 32(ABO 25 cases and Rh 7 cases)in total 41cases newborns who diagnosed as hemolytic disease were tested by the method of microcolum gel technology. Results 26 in 32 cases newborns had positive IgG1 or IgG3 synchronously. The concentration of IgG1 or IgG2 in 21 cases newborns was synchronously raised. There were 12 cases the IgG3 positive newborns developed hyperbilirubinemia and their bilirubin levels were significantly higher than that of only IgG1 positive neonates. However, there were 8 pregnant women with higher IgG2 or IgG4, their neonates were not discovered the symptom of hyperbilirubinemia. Conclusions There were positive relative for the blood type antibodies IgG subsets among the gravidaes and their neonates,especially IgG1 and IgG3 were important in pathogenesis of heamolytic disease of neonates caused by blood type. The IgG subsets detection was necessary for the high titer antibodies gravidaes. It might be helpful to early diagnose hyperbilirubinemia of newborns. The method could be provided a directional significance in early intervention of blood group incompatible hemolytic disease of the newborns and carried out an individual precise intervention treatment.

Objective To analyze the effect of red blood cell（RBC） washing procedureon direct antiglobulin test (DAT)by Microcolumn Gel Card, so as to improve the accuracy and reliability ofGel-DAT. Methods 78 Gel-DAT positive samples without washingwas detected by Gel again after three timeswashing. According tothe above experimental results, 78 samples were divided into group A (with changes after washing: the agglutination strength was±~3+), group B (with changes after washing: becamenegative) and group C (with no changes after washing). Group A andB were washed with room temperature normal saline (NS) for five times, then detected with Gelfor DAT. Group C was washed with 37℃ NS for five times, then Gel was incubated at 37℃ for 15 minutes for DAT.We recorded the Gel scoreson each time. Results RBC washing procedure had an effect on DAT Results 4 samples became negative, 3 samplesshowed no change before and after washing, and 71 samplesshowed more scores of agglutination strength after washing. In group A, it was a trend that agglutination intensity wasstrongest afterthe third time washing. In group C,there was no significant change after washing with 37℃ NS, but DAT became negative after incubation Gel on 37℃ for 15 minutes. Conclusion RBC washing procedure has an effect on Gel-DAT. It is suggested that patients' RBC samples should be washedbefore Gel-DAT.According to the clinical diagnosis and the specimen appearance, we select the corresponding washing frequencyand washing temperature, so as to improve the accuracy and reliability of experimental results.

Objective To investigate the effects of 4% sodium citrate, 4000U/mL heparin sodium and 6 250 U/mL heparin sodium on the critically ill hemofiltration patients after operation, and to analyze the relationship between the different locking solutions and the hemorrhage-related complications. Methods During our study period, 116 postoperation patients underwent bedside hemofiltration who were admitted to the intensive care unit (ICU) of Peking University First Hospital from February 2014 to July 2016, were analyzed. We conducted a retrospective cohort study. Compare the effectiveness and safety of use of 4% sodium citrate, 4 000 U/mL heparin sodium, 6 250 U/mL heparin on catheters locking. The results of the observation included blood loss index, postoperative blood loss, catheter patency, clotting function, and catheter infection rate 3 days and 10 days after surgery. Results A total of 116 patients received 439 post-hemofiltration closures during the study period. The blood loss index and significant blood loss at 3 and 10 days after sealing with 6 250 U/mL heparin sodium were significantly higher than those in the 4% citric acid group (P<0.001) and the 4 000 U/mL heparin sodium group (P<0.001). The result of PT test had no significantly different between the three groups of locking solutions, P=0.833. APTT was significantly different between the 4% citrate group and the 6 250 U/mL heparin group, P=0.023. The 4% citrate group had a higher rate of catheter dysfunction than the two heparin group by 6.91% vs4.92% vs2.98%, P>0.05, but not statistically significant. There was no difference in the rate of catheter related blood stream infection among the three groups of locking solutions, P>0.05. Conclusion 4% citrate and 4 000 U/mL heparin, 6 250 U/mL heparin locking solutions are equally effective in maintaining catheter patency in patients with hemofiltration. 6 250 U/mL heparin group had a higher risk of hemorrhage-related complications after surgery. 4% citrate group had a lower risk for systemic anticoagulation than 6 250 U/mL heparin group. The three locking solutions are similarly in catheter related blood stream infections.

Objective To develop an accurate blood transfusion specimen positioning information system. Methods The Department of Blood Transfusion of China-Japan Union Hospital of Jilin University and the Beijing Haihui Engineer jointly developed the precise specimen positioning function in the blood transfusion information management system. Results The intelligent management of blood transfusion specimens has been realized. From manual numbering and searching the donor specimens and blood bags one by one to accurate positioning of donor and patient specimens and blood components, accurate informatization management was achieved. Conclusions Based on the Haihui blood transfusion information management system, development the precise positioning function of blood transfusion specimens and blood components, which realized the intelligent working mode, improved the work efficiency of the blood transfusion department greatly, ensured the safety of blood transfusion.

Objective To strengthen and improve the comprehensive and effective performance management system and sustainthe healthy development of blood donation in organizations for the collection, manufacture and distribution of blood and blood products. Methods By means of issuing questionnaires, on-the-spot interviewsand expert consultation, we made a statistical analysis on the changing trend of blood collection volume, the relationshipbetween labor cost and the growth of blood collection, bloodcollectionefficiency per capita, staffing, implementation of performance pay and so on. Results Regular staff accounted for only 56.81% of the total. Professional and technical personnel accounted for 74.06% of the total number of post. 82.35% organizations adoptedperformance pay system. Since 2010, the blood collection volume has entered a platform and blood collection per capita efficiency has declined. There is a lack of unified, perfect and universally applicable performance evaluation system, the formulation of performance indicators is not scientific, performance evaluation methods are rough and so on. Conclusion Due to the imperfection of the system itself and many problems in the organization management, the advantages of performance management in the management of blood banks in China are not fully reflected. We should pay attention to top-level design, improve performance management and performance pay system, gradually increase the proportion of rewarding performance pay, trainperformance managers, and formulate scientific and reasonable performance evaluation indicators.

Objective To analyze the distribution characteristics of HLA-I loci (A, B) in apheresis platelet donors in Shijiazhuan area, explore the probability of finding an HLA-matched platelet donor in platelet donor pools in Shijiazhuang area, and evaluate the minimum pool size of platelet donor gene database. Methods HLA-I loci A and B genotypes of 1 935 unrelated platelet donors in Shijiazhuang area were performed by polymerase chain reaction(PCR) using sequence-specific primers (PCR-SSP) and sequence-based typing (PCR-SBT) methods. HLA genotype obtained were converted to antigenic specificity, calculated genetype frequency and haplotype frequency, and evaluated the minimum pool size of platelet donor gene database. The cross-reactive group (CREG) phenotype frequency and its mismatch rate were counted. Results A total of 19 HLA-A locus phenotype specificities were identified in 1 935 unrelated platelet donors, and the highest frequency antigens are A2 (0.313 4) and A11 (0.158 4). A total of 43 B-locus phenotype specificities were identified, and the highest frequency antigens are B13 (0.118 1) and B61 (0.086 3). 355 HLA-A-B haplotypes were observed, of which 28 frequencies were greater than 0.01, the highest frequencies haplotypes were A2-B13 (0.044 4) and A2-B62 (0.029 5). 1C-2C and 7C-5C have the highest mismatch rate of CREG epitopes of A-A and A-B antigens, and 4C-6C has the highest mismatch rate of Broad CREG in apheresis platelet donors in Shijiazhuang area. Conclusion In theory, 93.18% of patients have a 95% ;probability to find at least one HLA-matched donor in a platelet donor pool with a total storage capacity of 1,500 patients. The platelet donor pool size of 1935 would basically meet the needs of patients with platelet transfusion refractoriness in Shijiazhuang area.

Objective To investigate the genetic background of ABO blood group-incompatible specimens in 4 cases. Methods The blood group serological test was used to identify the subtypes of ABO positive and negative stereotyping inconsistency; PCR was used to amplify 7 exons of the ABO gene; the sequence analysis was directly performed for 7 exons. Results The blood group serology test indicated that 4 specimens were ABO subtypes, namely Bx (1 case); ABx(1 case); Ax (2 cases). The genes by direct sequencing revealed that Bx and ABx change were not detected in exons 1~6 on the molecular basis of ABO^*B.01, only the mutation at position 449A>G in exon 7 of B allele. The exons 1~6 change of 2 Ax cases were not detected on the molecular basis of ABO^*A1.01, only the mutation at position 467C>T and the insertion of a base T at position 798 in exon 7 of A allele. Changes in above gene loci resulted in changes in the serological phenotype of the ABO blood groups, which manifested as ABO subtype. Conclusion The study revealed the molecular genetic background of ABO subtypes in 4 cases. Two novel ABO alleles were found, namely c.449A>G single locus mutation, c.467C>T mutation and c.798insT single base insertion.

Objective To analyze the correlation of serum interferon-γ(IFN-γ), interleukin-2 (IL-2) and epidermal growth factor (EGF) for prediction of radiotherapy sensitivity and prognosis in patients with nasopharyngeal carcinoma. Methods Eighty six patients with nasopharyngeal carcinoma in the hospital were collected from 2010 to 2013. Simultaneously, sixty four healthy persons were selected as the control group basec on physical examination. Analysis of the relationship between serum IFN-γ, IL-2, and EGF and clinical pathological features,in both groups were compared. Results No significant difference of serum IFN-γ, IL-2 and EGF concentration in patients with nasopharyngeal carcinoma was noted in gender, ages, pathological types, differentiation types and clinical stages (P>0.05). The levels of IFN-γ and IL-2, however, in nasopharyngeal carcinoma group were lower but EGF was higher than those in the control group (P<0.05). High levels of serum IFN-γ and IL-2 and low levels of EGF in the radiotherapy sensitive group of patients with nasopharyngeal carcinoma were seen compared with those in the radiotherapy resistant group, (P<0.05). The Cox proportional hazard model showed that serum EGF level and radiosensitivity were risk factors for prognosis, and IFN-γ and IL-2 were protective factors. IFN-γ, IL - 2 and EGF areas under the curve were 0.719, 0.773 and 0.884, respectively. The joint detection area under the curve was 0.937, which was significantly higher than that of individual indicators. Conclusion The serum levels of IFN-γ, IL-2, EGF are associated with the development of nasopharyngeal carcinoma, indicating the potential significance of the cytokine detection in guidance of clinical treatments and prognosis of nasopharyngeal carcinoma.

Objective To investigate the expression and significance of drug resistance related genes O⁶-methylguanine-DNA-methyltransferase (MGMT) and DNA topoisomerase II (TopoII) in human glioma. Methods The expression of MGMT and TopoII in 96 cases of glioma tissues and 30 cases of normal brain tissues was detected by immunohistochemical staining method. The expression of genes was compared between different tissues, and the relationship between the expression of genes in glioma and clinical data and prognosis was analyzed. Results The positive expression rates of MGMT and TopoII in glioma tissues were 57.29% and 36.45%, respectively while in normal brain tissues were 6.33% and 3.33%, respectively (P<0.05). The expression rate of TopoII increased with the degree of tumor malignancy (P<0.05). 79 patients with gliomas were followed up for 3~32 months. The survival time of patients with negative of MGMT and TopoII was longer than that of patients with expression of MGMT and TopoII (P<0.05). Conclusion The expression rates of MGMT and TopoII are high in human glioma. The expression of TopoII is related to the malignant degree of glioma, and the expression of the two genes is related to the prognosis of patients.

Objective To study the correlation between serum epidermal cell-type fatty acid binding protein (E-FABP) and serum fibroblast growth factor 21 (FGF-21) and glucose and lipid metabolic indicators in patients with polycystic ovary syndrome. Methods 74 patients with polycystic ovary syndrome admitted to our hospital from April 2017 to March 2018 were enrolled. According to BMI, they were divided into obese group (n=43) and non-obese group (n=31), and 36 healthy subjects with similar BMI to patients in the same period were selected as the control group. The serum levels of FGF-21, E-FABP, glucose metabolism and lipid metabolism in the three groups were observed and compared. Results The serum FGF-21 and E-FABP levels of the obese group and the non-obese group were significantly higher than those of the control group (P<0.05), and the serum FGF-21 and E-FABP levels of the obese group were significantly higher than those of the non-obese group (P<0.05). The glucose metabolism level of the non-obese group and the obese group was significantly higher than that of the control group (P<0.05), and the glucose metabolism level of the obese group was significantly higher than that of the non-obese group (P<0.05). Non-obese group and obesity group patients LDL - C, FFA, TC and TG levels are significantly higher than control group (P<0.05), level of HDL - C in non-obese group and obesity group was obviously lower than that in the control group (P<0.05), LDL - C, FFA, TC and TG levels are significantly higher in obese patients than non-obese patients (P<0.05), HDL - C levels in patients with obesity group was obviously higher than non-obese patients (P<0.05); According to the data analysis, the serum FGF-21 and E-FABP levels were directly correlated with the glycolipid metabolism indicators (P<0.05). Conclusion According to the findings of this study, the serum levels of FGF-21 and E-FABP in patients with polycystic ovary syndrome can reflect the changes of patients' glucose and lipid metabolism, which is worthy of promotion and application.

Objective To observe the levels of serum homocysteine（Hcy） in the patients with maintenance hemodialysis （MHD）,and analysis of the affected factors related to homocystein. Methods There were 82 patients with maintenance hemodialysis （The dialysis duration is more than 3 months） were selected for this study. They were divided into high homocysteine group （59 cases,Hcy≥15μmol/L） and low homocysteine group （23 cases,Hcy<15μmol/L） according to the levels of serum homocysteine. The differences of biochemical index between the low homocysteine group and the high homocysteine group were compared, and the affected factors related to homocystein were analysed by Pearson correlation analysis/Logistic regression analysis. Result ①The average of the serum homocysteine is （42.15±7.28） μmol/L and the proportion of high homocysteine is 72%. ② Compared with the low homocysteine group,the levels of albumin, hemoglobin, high density lipoprotein and calcium in the high homocysteine were significantly decreased, but the levels of Low density lipoprotein, Fasting plasma glucose, Blood uric acid, Systolic blood pressure and Diastolic blood pressure were significantly increased. ③ Pearson linear correlation analysis show that the levels of homocysteine are positively correlation with the Low density lipoprotein, Fasting plasma glucose Blood uric acid, Systolic blood pressure and Diastolic blood pressure,while are negatively correlation with the albumin, high density lipoprotein and hemoglobin. Logistic regression analysis showed that albumin and hemoglobin were protective factors for homocysteine and the Low density lipoprotein, Fasting plasma glucose, Blood uric acid, Systolic blood pressure and Diastolic blood pressure were hazards factors for homocysteine. Conclusions The incidence of high homocysteine in patients with MHD was high;the albumin and hemoglobin were protective factors and the Low density lipoprotein, Fasting plasma glucose, Blood uric acid, Systolic blood pressure and Diastolic blood pressure were hazards factors for homocysteine.

Objective To investigate the clinical value of detection of HCV-cAg and its relationship with HCV-RNA, ALT and AST. Methods 112 HCV-cAg positive specimens and 205 HCV-Ab positive specimens were collected. Real-time Quantitative PCR method was used to detect HCV-RNA , and at the same time liver function (ALT, AST) was detected. Results There were 95 HCV-RNA-positive cases from the 112 HCV-cAg positive specimens ( a positive rate of 84.8%) and 142 HCV-RNA-positive cases from 205 HCV-Ab positive specimens (a positive rate of 69.3%), respectively, and therewas significantly higher HCV RNA positive rate in the former than that in the latter (P<0.05); The former had 70 cases of abnormal liver function (62.5%) and the latter 95 cases (46.3%). The former was significantly higher than that of the latter (P<0.05).The increase of the value of S/CO in HCV-cAg results was positively correlated with the positive rate of HCV-RNA and the abnormal rate of liver function. Conclusion The positive results of HCV-cAg test highly correlate to HCV-RNA, and reflect the status of liver function. HCV-Ag test has important value in assisted clinical diagnosis.

Objective To investigate the value of homocysteine (Hcy) in prognosis of the invasion of (UCs) myometrium in urothelial cell carcinoma. Methods The difference of Hcy between invasive and non-invasive UCs patients was analyzed in 57 patients with UCs; To analyze the difference of Hcy level between the patients with infiltrative UCs and those with non-myometrial infiltration; The level of Hcy was used to determine whether UCs has myometrial infiltration or not to draw the operating curve of subjects ROC; Analysis of the relationship between myometrial infiltration and other pathological parameters in patients with UCs. Results The difference of Hcy between invasive and non-invasive UCs patients was statistically significant (t=2.103, P=0.04); There was significant difference in Hcy between UCs patients with myometrial infiltration and without(t=3.109, P=0.003); The Hcy value of 11.95 can be used as the best cut-off point for myometrium infiltration in UCs patients; Myometrial infiltration in UCs patients was correlated with age≥70 and Hcy level (P<0. 05); Conclusion The level of Hcy can helpful to judge whether there is myometrial infiltration in UCs patients.

Objective To investigate the clinical value of blood cell count, C-reactive protein and novel coronavirus (SARS-CoV-2) specific IgM antibody in the diagnosis of COVID-19. Methods The peripheral blood leukocyte, lymphocyte counts and C-reactive protein in 59 patients diagnosed with COVID-19 were detected and analyzed,and the level of SARS-CoV-2 specific IgM antibody in serum of patients with different course of disease were detected by colloidal gold immunochromatography. Results ①Among the 59 patients, 57 (96.61%) had normal or decreased leukocyte count,20 (33.9%) had decreased lymphocyte count and 41 (69.49%) had increased C-reactive protein.Compared with the healthy people, there were significant differences in the counts of leukocytes, lymphocytes and C-reactive protein in the patients with COVID-19（P< 0.05）. ②Ofthe 59 confirmed patients, 52 (88.14%) had positive SARS-CoV-2 specific IgM antibody in their serum, while the healthy subjects had negative IgM antibody. In patients with COVID-19, the positive rates of serum SARS-CoV-2 specific IgM antibodies were 25%, 81.82%, 90%, and 100% during the course of 0-6 d, 7-10 d, 11-15 d, 16-20 d, respectively. ③There was no significant difference in the levels of peripheral blood leukocytes, lymphocytes and C-reactive protein between the IgM positive group and the IgM negative group (P>0.05). Conclusion ①After infection with SARS-CoV-2,the leukocyte count in peripheral blood was generally normal or decreased,and some patients presented lymphocytopenia, often accompanied by elevated C-reactive protein.Dynamic observation of the changes of leukocytes, lymphocytes and C-reactive protein is of great significance for the clinical diagnosis and disease observation of COVID-19.②The detection of SARS-CoV-2 specific IgM antibodies in the serum of patients by colloidal gold immunochromatography can be used for auxiliary diagnosis of patients with SARS-CoV-2 infection.

Objective To investigate the relationship between the level of erythropoietin producing hepatocyte receptor （Eph） A5 in sera and placenta tissues and pregnant outcomes in patients with placenta previa （PP）. Methods Ninety-eight patients with PP in the hospital were selected as observation group. Patients with complete, partial and marginal PP were included in groups A, B and C according to PP typing. Simultaneously, 40 healthy pregnant women were taken as the control. The prenatal blood samples and postpartum placenta tissues were collected in the observation groups and control . The level of Eph A5 in the sera and placental tissues of the all subjects were detected and followed up for occurrence of adverse pregnant outcomes. Results The level of Eph A5 in the sera and placentas of the observation groups was lower than that of the control （P<0.05）, much lower in groups A and B than group C （P<0.05）, but no significant difference was seen between group A and group B （P>0.05）. The incidence of adverse pregnant outcomes in maternal and perinatal children were 59.18% and 41.84%, respectively. The serum and placental Eph A5 level in the group of severe abnormal pregnant outcomes decreased compared with that of the mild ones （P<0.05）. No significant difference was noted of maternal serum Eph A5 among perinatal abnormal pregnancies （P>0.05）. Conclusion Eph A5 level in serum and placental tissues of patients with PP abnormally decreased, especially in patients with complete and partial placenta previa. The decreased level of serum Eph A5 is associated with maternal adverse pregnancies whereas Eph A5 in placental tissue is related to adverse fetal outcomes.

Objective To explore the application effect and value of enhanced safety nursing based on evidence-based concept in patients with severe renal anemia during transfusion. Methods The clinical data of 62 patients with severe renal anemia who received routine nursing and 70 patients who received intensive safety nursing based on evidence-based concept were retrospectively analyzed and recorded as routine nursing group, intensive nursing group. The nursing effect and nursing satisfaction were compared between the two groups. Results After nursing, the scores of BAI and BDI in both groups decreased （P<0.05）, while those in intensive nursing group were lower than those in routine nursing group （P<0.05）. After intensive nursing, the correct rates of health cognition about the content of application form, application process, common blood products, the difference between infusion and infusion, indication, effect evaluation, adverse reactions, emergency measures for adverse reactions and relevant legal knowledge in intensive nursing group were higher than those in routine nursing group （P<0.05）. The incidences of hemolysis, fistula blockage and nosocomial infection in intensive nursing group were lower than those in routine nursing group （P< 0.05）. There was a significant difference in nursing satisfaction grade between intensive nursing group and routine nursing group （P<0.05）, and the total satisfaction rate was higher than that of the latter （P<0.05）. Conclusion Enhanced safety nursing based on evidence-based concept in patients with severe renal anemia transfusion can not only ensure the safety of blood transfusion, improve health awareness and improve nursing satisfaction, but also reduce negative emotions.