Abstract: Objective To analyze the correlation between miR-149-5p and phosphoglycerate dehydrogenase （PHGDH）in chronic myeloid leukemia（CML）imatinib（IM）resistance and glycolytic activity. Methods The expression of miR-149-5p and PHGDH,and glucose uptake in CML cell line K562 and imatinib-resistant CML cell line K562G were detected. The overexpression of MiR-149-5p was obtained in 149G and control 149N from K562G cells by lentiviral transduction. The expression of miR-149-5p and PHGDH,and glucose uptake in 149G and 149N cells were detected. The Liquid chromatography with tandem mass spectrometry（LC-MS-MS）technology was used to target metabolomics analysis. Results The expression level of miR-149 in imatinib-resistant CML cell line K562G was significantly lower than that in imatinib-sensitive CML cell line K562（P<0.05）. The expression of PHGDH was significantly increased in K562G（P<0.05）. Compared with 149N cells, the expression level of miR-149 in 149G was significantly increased （P<0.05）,while the expression of PHGDH was significantly decreased（P<0.05）. The expression of glucose uptake in K562G was higher than that in K562（P<0.05）. Compared with 149N cells,the glucose uptake of 149G was reduced （P<0.05）. Metabolomics analysis showed that there were 9 metabolites significant differences between K562 and K562G （P<0.05）. Conclusion Expression miR-149-5p was associated with reprogramming glucose metabolism in CML cells, and overexpression miR-149-5p could target downstream inhibition of PHGDH expression. MiR-149-5p targeting the PHGDH pathway may inhibit glycolytic activity in CML cells.

Key words: MiR-149-5p, 3-Phosphoglycerate dehydrogenase, Chronic myeloid leukemia, Imatinib, Liquid chromatography with tandem mass spectrometry