Identification of ABO Elution Subtypes by Genotyping Technology

doi:10.3969/j.issn.1671-2587.2022.02.002

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2022, Vol. 24 ›› Issue (2): 142-146.DOI: 10.3969/j.issn.1671-2587.2022.02.002

Previous Articles Next Articles

Identification of ABO Elution Subtypes by Genotyping Technology

ZHANG Ruo-yang, YANG Dong, FENG Chen-chen, et al

Department of Transfusion Research, Jiangsu Province Blood Center, Nanjing 210042

Received:2021-09-30 Published:2022-04-12

Abstract

Abstract: Objective To explore the safe blood transfusion strategy of Ael/Bel subtypes, sequence analysis of ABO genes in 10 samples for which the reason for serological phenotype or suspected elution subtype was conducted. Methods ABO phenotypes of ten samples were identified by serological methods. The exons 1~7,the upstream CBF/NF-Y enhancer region,promoter and the +5.8 kb erythroid cell-specific regulatory elements in ABO intron 1 genes were amplified, sequenced and analyzed. Results The serological phenotypes were identified or suspected of elution subtypes ABO gene sequences analysis showed that the genotypes of five AEL cases were ABO*AEL.02/O.01.01（2 cases）,ABO*AEL.08/O.01.01（1 case）and ABO*AEL.02/O.01.02（2 cases）,respectively; all five BEL cases were ABO*BEL.03/O.01.02. Conclusion Elution subgroup is difficult to be detected by routine serological methods. It can be accurately identified by molecular biology techniques. It is helpful to guide safe and effective blood transfusion strategies.

Key words: Elution subtypes, Genotyping, Safe transfusion

CLC Number:

R457

ZHANG Ruo-yang, YANG Dong, FENG Chen-chen, et al. Identification of ABO Elution Subtypes by Genotyping Technology[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(2): 142-146.

References

[1] STORRY J R,OLSSON M L.The ABO blood group system revisited:A review and update[J]. Immunohematology,2009,25(2):48-59.
[2] 尚红,王毓三,申子瑜,等.全国临床检验操作规程[M].第4版.北京:人民卫生出版社,2015:118-124.
[3] CHEN Q,XIAO J,WANG S,et al.ABO sequence analysis in an AB type with anti-B patient[J].Chin Med J (Engl),2014,127(5):971-972.
[4] FENG C,LI H,LIU T,et al.ABO*B.01+c.464A>C represents a missense variation in the ABO gene and encodes a weak B phenotype[J]. Transfusion,2021,61(9):E59-E61.
[5] DANIELS G.Human blood groups[M].3rd.Oxford,UK:Wiley-Black-well,2013:11-19.
[6] BENNETT EP,STEFFENSEN R,CLAUSEN H,et al.Genomic cloning of the human histo-blood group ABO locus[J].Biochem Biophys Res Commun,1995,206(1):318-325.
[7] SELTSAM A,HALLENSLEBEN M,KOLLMANN A,et al.The nature of diversity and diversification at the ABO locus[J].Blood,2003,102(8):3035-3042.
[8] YAMAMOTO F,CLAUSEN H,WHITE T,et al.Molecular genetic basis of the histo-blood group ABO system[J]. Nature,1990,345(6272):229-233.
[9] YAZER MH,OLSSON ML,PALCIC MM.The cis-AB blood group phenotype: fundamental lessons in glycobiology[J].Transfus Med Rev,2006,20(3):207-217.
[10] OLSSON ML,CHESTER MA.Heterogeneity of the blood group Ax allele:genetic recombination of common alleles can result in the Ax phenotype[J]. Transfus Med,1998, 8(3):231-238.
[11] 吕杭军,洪小珍,应燕玲,等.ABO血型新等位基因Ael08的分子生物学研究[J].中国输血杂志,2011,24(8):676-679.
[12] 章旭,李剑平.α-1,3-N-乙酰半乳糖胺转移酶基因467C>T和804insG变异导致Ael亚型[J].中华医学遗传学杂志,2016,33(4):526-529.
[13] 龚淞颂,邹纬,李芳,等.B糖基转移酶p.R168W突变导致Bel亚型的分子机制研究[J].中国输血杂志,2017,30(7):679-681.
[14] HOSSEINI-MAAF B,IRSHAID NM,HELLBERG A,et al.New and unusual O alleles at the ABO locus are implicated in unexpected blood group phenotypes[J]. Transfusion,2005,45(1):70-81.
[15] 骆宏,张润青,魏玲,等.中国南方汉族人群特殊O型个体的血清学特征和分子背景[J].中山大学学报(医学版),2018,39(4):521-525.
[16] 骆宏,张润青,廖志坚,等.特殊A/O基因型与O表型的关系[J].中华医学遗传学杂志,2019, 36(3):275-277.
[17] OLSSON ML,MICHALEWSKA B,HELLBERG A,et al.A clue to the basis of allelic enhancement: occurrence of the Ax subgroup in the offspring of blood group O parents[J]. Transfus Med,2005,15(5):435-442.
[18] CHO D,SHIN MG,YAZER MH,et al.The genetic and phenotypic basis of blood group A subtypes in Koreans[J]. Transfus Med,2005,15(4):329-334.
[19] 兰炯采,陈静娴,马红丽,等.推荐ABO疑难血型三步分析法[J].中国输血杂志,2010,23(3):165-168.
[20] 孙嘉峰,杨晓俊,张爱,等.3例B亚型标本的血清学表型和基因型结果分析[J].临床输血与检验,2020,22(5):506-509.
[21] 何卫社,梁俊杰,刘建岭,等.罕见患者AEL04亚型1例及其家系调查[J].临床输血与检验,2017,19(2):203-204.

Identification of ABO Elution Subtypes by Genotyping Technology

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments

About

Author

Journal

Access Statistics

Contact

[1]	ZHAO Yang, HU Xiao-yu, LV Rong, et al. HPA Genotyping of Blood Samples from Apheresis Platelet Donors in Hefei,Anhui Province [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(1): 74-79.
[2]	CHEN Wang, LIU Ai-ling, WU Min. Natural Resistance of HBV to LMV/ADV Treatments and Its Association with Genetic Structure in Shenzhen Region [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(6): 619-622.
[3]	LIANG Zhi-hong, XIE Jing-wen, RUAN Li-fen, et al. RHD Gene Genotyping in Pregnant Women with Rare Blood Type [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(4): 348-351.
[4]	ZHU YU-Li, GAO Xiang-yang, LIU Li. A Case Report Of Di(a+b-) Blood Group with Anti-Dib Antibody [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(4): 438-441.
[5]	TAN Wei. Analysis of RhD Phenotype and Genotype in RhD （-）Blood Donors [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2017, 19(6): 621-623.
[6]	ZHAO Ling-jun. A Case Report of Forward/Reverse Blood Grouping Discrepancy Resulting from ABO Gene Variant [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2017, 19(2): 178-182.
[7]	LV Ran, LIU Yu, WANG Peng-rui, et al. Xingtai. Establishment of HPA Gene Database for Platelet Donors in Xingtai Area of Hebei [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2017, 19(1): 55-58.
[8]	CHEN Qing, LI Ping, LU Le, et al. Identification of Weak D Type 15 of Rh Blood Group System [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2016, 18(5): 416-419.