Abstract: Objective Long-term use of large-dose caffeine sodium benzoate, a psychotropic drug, has a great impact on the physical and mental health of users, leading to not only addiction but also varieties of diseases. This study was to investigate the mechanism of endothelial cell dysfunction mediated by caffe natrium causing coagulation dysfunction. Methods Based on clinical data, the effects of sodium ampoule on coagulation function and related diseases were examined. β-galactosidase staining was done to observe the stimulation of human umbilical vein endothelial cells (HUVECs) using 25% sodium cafe patient serum +75% normal human serum, 50% sodium cafe patient serum +50% normal human serum, 75% sodium cafe patient serum +25% normal human serum, sodium cafe patient serum and lipopolysaccharide, and the effects of sodium ampoule serum on HUVECs were analyzed via the HUVEC cell tube experiment to test the ability of cells to form blood vessels. Results Long-term consumption of caffeine sodium benzoate seriously affected the coagulation function of patients, and the proportion of positive cells increased with higher coffee with sodium ampna serum (P<0.05), i.e. senescent cells were elevated, and cell tube experiments showed that HUVECs blood vessel formation rate decreased after serum stimulation in patients with caffeine sodium benzoate (P<0.05). Conclusion Long-term consumption of caffeine sodium benzoate can lead to abnormal function of vascular endothelial cells in patients and hence coagulation dysfunction.

Key words: Caffeine sodium benzoate, Vascular endothelial cell, Coagulation disorders