Comparative Study on the Protective Effect of Different Preservation Solutions on Red Blood Cell Storage Lesion in Type 2 Diabetes Mellitus

doi:10.3969/j.issn.1671-2587.2023.06.001

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2023, Vol. 25 ›› Issue (6): 721-726.DOI: 10.3969/j.issn.1671-2587.2023.06.001

Comparative Study on the Protective Effect of Different Preservation Solutions on Red Blood Cell Storage Lesion in Type 2 Diabetes Mellitus

YOU Laiwei, YAO Na, WU Mandi, et al

Department of Anesthesiology, Gongli Hospital, Naval Military Medical University, Shanghai 200135

Received:2023-09-26 Online:2023-12-20 Published:2024-01-15

Abstract

Abstract: Objective To investigate the effects of different formulas of red blood cell (RBC) preservation solutions on RBC storage lesions in vitro to provide optimal storage solution for RBC in patients with type 2 diabetes mellitus (T2DM). Methods A total of 20 diabetic patients and 20 non-diabetic patients who underwent elective surgery from June 2021 to January 2022 were collected. RBCs were stored in different preservation solutions (preservation solution a, preservation solution b and preservation solution c) to select out the appropriate preservation solution (DO-o). Morphologic and metabolic changes in RBCs were observed in the conventional preservation solution of non-diabetic patient group (NC group), the conventional preservation solution and the new preservation solution of diabetic patient group (DO-c group and DO-o group). Results At T0 (immediately after red blood cell separation), T1 (storage for 7 days), T2 (storage for 14 days), T3 (storage for 21 days) and T4 (storage for 28 days),RBC count, hemoglobin, Na⁺ concentration and pH value in each group gradually decreased (P<0.05), while K⁺ concentration gradually increased (P<0.05). The RBC count in preservation solution group b was significantly higher than that in group a and c (P<0.05). At T2, T3 and T4, the levels of hemoglobin in group a and b were higher than that in group c (P<0.05). At T3 and T4, Na⁺ in group b was higher than that in the other two groups (P<0.05). At T2, T3 and T4, K⁺ in group a and c was lower than that in group b (P<0.05). At each time, there were statistically significant differences in hemolysis rate, Free Hemoglobin (FHb), Lactic Acid (LA) and Lactate Dehydrogenases (LDH) within and between groups (P<0.05); the hemolysis rate, FHb, LA and LDH were significantly lower than those in the DO-c group (P<0.05). Adenosine Triphosphate(ATP) in all groups gradually decreased, and the difference was statistically significant (P<0.05); at T1, T2 and T4, ATP in DO-o group was higher than that in DO-c group (P<0.05). Conclusion The new preservation solution can significantly improve RBC storage lesions in diabetic patients.

Key words: New preservation solution, Type 2 diabetes, Red blood cell storage lesions, pH, NaCl, Glucose

CLC Number:

R457

YOU Laiwei, YAO Na, WU Mandi, et al. Comparative Study on the Protective Effect of Different Preservation Solutions on Red Blood Cell Storage Lesion in Type 2 Diabetes Mellitus[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(6): 721-726.

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Comparative Study on the Protective Effect of Different Preservation Solutions on Red Blood Cell Storage Lesion in Type 2 Diabetes Mellitus

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