Application of ONT in Ambiguous Results of the HLA-DPB1 Genotyping

doi:10.3969/j.issn.1671-2587.2024.03.011

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2024, Vol. 26 ›› Issue (3): 365-370.DOI: 10.3969/j.issn.1671-2587.2024.03.011

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Application of ONT in Ambiguous Results of the HLA-DPB1 Genotyping

LIAN Xue, ZHANG Dan, WEN Yujie, FAN Chengyan, WANG Dongmei, LIU Na, ZHANG Rongfang, JIA Yanjun

HLA Laboratory, Beijing Red Cross Blood Center, Beijing 100088

Received:2024-03-05 Online:2024-06-20 Published:2024-06-24

Abstract

Abstract: Objective This study aims to analyze the efficiency, advantages and mechanisms of the ONT platform, a TGS method in ambiguous results of the HLA-DPB1 genotyping in comparison to PCR-SBT and NGS. Methods 48 samples were tested for HLA-DPB1 genotyping by PCR-SBT, NGS（Illumina platform）and TGS (ONT platform). HLA-DPB1 allele was assigned to the third field for NGS and TGS, and the second field for PCR-SBT. Results TGS had no ambiguity with a single allele combination of HLA-DPB1 alleles. Ambiguous results were given in 45 out of 48 samples by PCR-SBT, accounting for 93% (45/48), and 5 out of 48 samples by NGS, accounting for 10% (5/48). Conclusion Compared with NGS and PCR-SBT, TGS can resolve most of the ambiguous combinations and improve the efficiency and accuracy in HLA-DPB1.

Key words: Human leukocyte antigen, PCR-SBT, NGS, TGS, ONT

CLC Number:

R446

LIAN Xue, ZHANG Dan, WEN Yujie, FAN Chengyan, WANG Dongmei, LIU Na, ZHANG Rongfang, JIA Yanjun. Application of ONT in Ambiguous Results of the HLA-DPB1 Genotyping[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(3): 365-370.

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Application of ONT in Ambiguous Results of the HLA-DPB1 Genotyping

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