Inhibitory Effects of Hydrogen on Activation and Apoptosis in Stored Apheresis Platelets

doi:10.3969/j.issn.1671-2587.2025.06.005

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2025, Vol. 27 ›› Issue (6): 775-782.DOI: 10.3969/j.issn.1671-2587.2025.06.005

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Inhibitory Effects of Hydrogen on Activation and Apoptosis in Stored Apheresis Platelets

LI Jinqi¹, QIN Aihua¹, REN Yufei², TANG Heshan¹, CHEN Cuimin², QIAN Baohua¹, ZANG Yan¹

¹Department of Transfusion Medicine, First Affiliated Hospital of Naval Medical University, Shanghai 200433;
²Clinical Research Center of the First Affiliated Hospital of Naval Medical University, Shanghai 200433

Received:2025-03-25 Published:2025-12-24

Abstract

Abstract: Objective To explore the effect and mechanism of hydrogen (H₂) on apheresis platelets activation and apoptosis. Methods 10 apheresis platelets were divided equally into 16 small bags and randomly divided into an H₂ group and a control group. The H₂ group was added with hydrogen to saturation and supplemented with hydrogen daily. All samples were stored under agitation at (22±2) ℃ for 8 days, the platelet count was tested, and the CD62P, PAC-1, apoptosis were detected by flow cytometry daily. The Caspase-3, Caspase-9, Bax, and Bcl-2 proteins were detected by Western blotting. Results There was no significant difference in platelet count during the preservation period (1~8 d). From the fifth day, the apoptosis rate in H₂ group was significantly lower than that in the control group (20.29±4.32 vs 33.06±8.01, P<0.001). From the sixth day, the expression of CD62P in H₂ group was lower than that in the control group (24.94±5.53 vs 47.14±10.41, P<0.001), and the expression of PAC-1 in H₂ group was lower than that in the control group (14.89±5.98 vs 19.41±5.17, P<0.05). Western blotting results showed that from the sixth day, the expression of cleaved Caspase-9 and cleaved Caspase-3 in the H₂ group was lower than that in the control group (P<0.001). From the seventh day, the expression of Bax in the H₂ group was lower than that in the control group (P<0.001), while Bcl-2 was higher than that in the control group (P<0.05), the ratio of Bax/Bcl-2 was lower than that of the control group. Conclusion Hydrogen can reduce platelet activation and apoptosis, improve the quality of apheresis platelet storage.

Key words: Hydrogen, Platelet storage lesion, Activation, Apoptosis

CLC Number:

R331.1⁺43

LI Jinqi, QIN Aihua, REN Yufei, TANG Heshan, CHEN Cuimin, QIAN Baohua, ZANG Yan. Inhibitory Effects of Hydrogen on Activation and Apoptosis in Stored Apheresis Platelets[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(6): 775-782.

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Inhibitory Effects of Hydrogen on Activation and Apoptosis in Stored Apheresis Platelets

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