Antibody Titration for Antibody Specificity in Patients with Autoimmune Hemolytic Anemia

doi:10.3969/j.issn.1671-2587.2022.01.014

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2022, Vol. 24 ›› Issue (1): 63-66.DOI: 10.3969/j.issn.1671-2587.2022.01.014

Previous Articles Next Articles

Antibody Titration for Antibody Specificity in Patients with Autoimmune Hemolytic Anemia

Gao Ming, Jin Sha, Xiang Dong, et al

Department of Blood Transfusion,South Hospital of the Sixth Affiliated People's Hospital of Shanghai Jiao Tong University,Shanghai 201499

Received:2021-07-24 Published:2022-01-27

Abstract

Abstract: Objective To identify specific antibodies（allo-antibody and mimicking antibody）in patients with autoimmune hemolytic anemia（AIHA）by antibody titration procedure. Methods Samples from patients tested positive for direct antiglobulin test from the blood group reference laboratory of Shanghai Blood Center between March and April 2021 were collected. Serum,eluate and diluent eluate for antibody titers were used to identify antibodies. Results Four specific antibodies in serum,3 specific antibodies in eluate,9 specific antibodies in diluent eluate were detected. Conclusion The proportion of specific antibodies detected in diluent eluate in positive DAT patients were higher than that of serum and eluate. The antibody titers play a decisive role in eluate dilution. Testing for antibody titer can increase detection rate of mimicking antibody in AIHA patients. It can reduce the reduction of blood transfusion effect due to mimicking antibody to improve the safety and effectiveness of a transfusion.

Key words: Eluate Antibody titer, Allo-antibody, Mimicking antibody

CLC Number:

R457

Gao Ming, Jin Sha, Xiang Dong, et al. Antibody Titration for Antibody Specificity in Patients with Autoimmune Hemolytic Anemia[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(1): 63-66.

References

[1] 段秉政,王淑莲,连俊慧,等. 直接抗人球蛋白试验在患者输血疗效中的临床意义分析[J].临床输血与检验,2021,23(2):189-191.
[2] ISSITT P D,COMBS M R,BUMGARNER D J,et al.Studies of antibodies in the sera of patients who have made red cell autoantibodies[J]. Transfusion,1996,36(6):481-486.
[3] DWYRE D M,CLAPPER A,HEINTZ M,et al.A red blood cell autoantibody with mimicking anti-E specificity[J]. Transfusion,2004,44(9):1287-1292.
[4] 田冬冬,赵鼎,李志浩,等.罕见抗LW抗体自身免疫性溶血性贫血合并新生儿溶血病患者的输血策略[J].中国实验血液学杂志,2021,29(06):1935-1939.
[5] MINAKAWA K,OHTO H,YASUDA H,et al.Efficacy of D-red blood cell transfusion and rituximab therapy in autoimmune hemolytic Anemia with anti-D and panreactive autoantibodies arising after hematopoietic stem cell transplant[J]. Transfusion,2018,58(7):1606-1610.
[6] BAJPAI M,MAHESHWARI A,GUPTA S,et al.Autoanti-C in a patient with primary sclerosing cholangitis and autoimmune hemolytic Anemia:a rare presentation[J]. Immunohematology,2016,32(3):104-107.
[7] HSIEH H Y,MORONEY D L,NAUMANN D E,et al.Warm autoimmune hemolytic Anemia with mimicking anti-c and-E specificities[J]. Immunohematology,2020,18(1):19-22.
[8] DATTA S S,REDDY M,BASU S.Warm autoimmune hemolytic Anemia with mimicking anti-e specificity causing intravascular hemolysis in a chronic ITP patient[J]. Transfus Apher Sci,2015,53(2):205-207.
[9] GARRATTY G,SATTLER M S,PETZ L D,et al.Immune hemolytic Anemia associated with anti-Kell and a carrier state for chronic granulomatous disease[J]. Rev Fr Transfus Immunohematol,1979,22(5):529-549.
[10] MANNY N,LEVENE C,SELA R,et al.Autoimmunity and the kell blood groups:auto-anti-kpb in a kp(a+b-) patient[J]. Vox Sang,1983,45(3):252-256.
[11] SULLIVAN C M,KLINE W E,RABIN B I,et al.The first example of autoanti-Kx[J]. Transfusion,1987,27(4):322-324.
[12] VIGGIANO E,CLARY N L,BALLAS S K.Autoanti-K antibody mimicking an alloantibody[J]. Transfusion,1982,22(4):329-332.
[13] WIN N,KAYE T,MIR N,et al.Autoimmune haemolytic anaemia in infancy with anti-Kpb specificity[J]. Vox Sang,1996,71(3):187-188.
[14] GOLDFINGER D,ZWICKER H,BELKIN G A,et al.An autoantibody with anti-wrb specificity in a patient with warm autoimmune hemolytic Anemia[J]. Transfusion,2003,15(4):351-352.
[15] GRISHABER J E,CORDLE D G,STRAUSS R G.Development of alloanti-Jka in a patient with hemolytic Anemia due to autoanti-Jkb[J]. Am J Clin Pathol,1992,98(5):542-544.
[16] GUPTA A,POKHREL A,SACHDEVA P,et al.Rare case of Auto immune haemolytic anaemia in an infant with auto “f & Kidd antibodies”[J]. Transfus Apher Sci,2020,59(4):102762.
[17] BOWMAN H S,MARSH W L,SCHUMACHER H R,et al.Auto anti-N immunohemolytic Anemia in infectious mononucleosis[J].Am J Clin Pathol,1974,61(4):465-472.
[18] JOHNSON MH, PLETT MJ,CONANT CN et al. Autoimmune hemolytic anemia with anti-S specificity[J]. Transfusion,1978,18(3): 389.
[19] HARRIS T.Two cases of autoantibodies that demonstrate mimicking specificity in the Duffy blood group system[J]. Immunohematology,1990,6(4):87-91.
[20] KLEIN H G,ANSTEE D J.Mollison's blood transfusion in clinical medicine[M].Oxford,UK:Blackwell Science Ltd,2005.
[21] MOLLISON P L.Measurement of survival and destruction of red cells in haemolytic syndromes[J]. Br Med Bull,1959,15(1):59-67.

Antibody Titration for Antibody Specificity in Patients with Autoimmune Hemolytic Anemia

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

About

Author

Journal

Access Statistics

Contact

[1]	ZHANG Rong, WANG Wen-ting, GU Shun-li, et al. Discussion on Monitoring Process and Blood Transfusion Strategy of Passenger Lymphocyte Syndrome [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 681-685.
[2]	WANG Qian, YANG Li-hui, DING Tian-ling, et al. Clinical Analysis of Platelet Transfusion for Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation with Different Conditioning Regimens [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 686-692.
[3]	. [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 693-695.
[4]	. [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 696-699.
[5]	FU Xiao-yan, ZHANG shuai-hang, SHAO hua, et al. Impact of Erythrocyte Storage Duration on Blood Indexes in Children with Open Heart Surgery by Cardiopulmonary Bypass [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 700-703.
[6]	QIU Li-juan, FU Xiao-yan, GUO Kai, et al. Analysis of Antibody Titers of 60 Pediatric Patients with Strong Positive Platelet Antibody Screening Results [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 704-707.
[7]	WANG Cui-e, LIU Zhi-wei, KONG Chang-hong, et al. Establishment and Application of Clinical Transfusion Service Information Platform [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 715-718.
[8]	JING Yuan-yuan, WANG Jie, LI Dong-mei, et al. Factors Influencing Development of HLA and/or HPA Antibodies in Patients with Platelet Refractoriness [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 723-727.
[9]	MA Ting, NIU Jia-meng, CHEN Xin-yue, et al. Analysis of Blood Transfusion in 8 053 Hospitalized Deceased Patients [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 732-736.
[10]	WANG Rui-han, LIU Hao, LIU Pei, et al. Indication of Intraoperative Cell Salvage in Cesarean Section [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 742-745.
[11]	MA Ling, WU Min-hui, ZHAO Fang, et al. The Genotyping and Alloimmunization Study of RhD among Perinatal Women with RhD Initial Typing Negative [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 746-749.
[12]	. [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 794-796.
[13]	. [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 800-802.
[14]	. [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 803-805.
[15]	. [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 806-810.