Abstract: Objective To identify irregular antibodies in a patient with a history of blood transfusion six months prior and the presence of DAT. Methods Two sets of antibody screening cells and two sets of screening panel cells were utilized to screen and identify irregular antibodies. Capillary ultra-high-speed centrifugation was employed to separate new and old cells, thereby determining the positive type of direct anti-human globulin. Antibody identification was subsequently performed in an acid release solution, and antibody specificity was determined in conjunction with patient antigen typing. Results Anti-Di^a and anti-Wr^a antibodies were detected in the patient. Distal DAT typing was positive for anti-IgG1 and negative for anti-C3d. Proximal DAT typing was negative for both anti-IgG and anti-C3D. Anti Di^a and anti-Wr^a antibodies were detected in the release fluid. Conclusion The patient exhibited the presence of anti-Di^a and anti-Wr^a antibodies. These antibodies were confirmed in the release fluid, indicating that the patient produced identical anti-Di^a and anti-Wr^a alloantibodies.

Key words: Diego Blood group system, Anti-Di^a, Anti-Wr^a, Alloantibody