Abstract: Objective To investigate the relationship between anti-HCV,HCV RNA and ALT in volunteer blood donors. Methods Fluorescent quantitative PCR was used to detect viral loads and kinetic method was employed to assay ALT levels in 235 anti-HCV positive samples who were tested by ELISA. A six month follow up was performed to monitor the anti-HCV（+）/HCV RNA（-） samples. Results Among 235 anti-HCV positive samples,140（59.57%） were positive for HCV RNA. The HCV RNA positive rate in groups of S/CO ratio 1~5 and 5.01~9.99 were 34.29% and 26.47%, respectively. The HCV RNA positive rate in group of S/CO ratio≥10 was 81.68% and statistically significant differences were found between this and the other two groups（χ² =45.15,P<0.05;χ²=39.41,P<0.05）. The ALT abnormality rates of anti-HCV（+）/HCV RNA（+） and anti-HCV（+）/HCV RNA（-） groups were 2.86% and 1.05%, respectively. No statistically significant difference was found between them（χ² =0.88,P≥0.05） . The age of donors with anti-HCV（+）/HCV RNA（+）were older than those with the anti-HCV（+）/HCV RNA（-) （χ² =7.81,P<0.05）. Among 95 blood donors,23 received retrospective survey and the anti-HCV results of one sample was negative in the second detection. Conclusion The positive rate of HCV RNA has relationship with the S/CO ratio of anti-HCV and the age of HCV infected blood donors. No correlation was found between the unqualified rate of ALT and HCV RNA in anti-HCV positive volunteer blood donors.

Key words: Volunteer blood donors, Anti-HCV, HCV RNA