Abstract: Objective To explore the clinical application value of the three parameter in the diagnosis and treatment of hepatitis B virus infection ,we analysed the relationship between pre S1 antigen (PreS1), hepatitis B virus nucleic acid (HBV-DNA) and hepatitis B virus serum markers (HBV-M). Methods A retrospective analysis was conducted on the detection data of PreS1, HBV-DNA and HBV-M in 1 184 patients with suspected HBV infection in our hospital from January 2016 to June 2018. Results There was a statistical difference between the PreS1 and the other six groups (χ²=87.597,111.736,216.866,168.015,421.362,324.532,202.062, P<0.05), and the positive rate of HBV-DNA in the "big three positive" group was significantly different from that of the other six groups (χ²=131.174,254.110,216.866,158.416,402.563,306.883. 181.859, P<0.05), the positive rate of PreS1 and HBV-DNA was much higher than that of other groups, and there was a significant association between PreS1 and HBeAg (χ²=478.981, P<0.001), and there was a significant correlation between PreS1 and HBV-DNA (χ²=909.450, P<0.001),the diagnostic coincidence rate between PreS1 and HBeAg was 85.13% and that between pre and HBV-DNA was 94.93%. the average viral load of HBV-DNA in patients with positive hepatitis B was higher than that in HBeAg negative hepatitis B patients (P<0.05). Conclusion the traditional serological detection of hepatitis B virus (HBV) HBeAg has certain correlation with PreS1 and HBV-DNA. PreS1 and HBV-DNA can more accurately reflect the replication status and contagion of HBV.

Key words: Pre S1 antigen, Hepatitis B virus nucleic acid, Hepatitis B virus serum markers