The Influence of Rapamycin on NF-κB p65 and STAT-1 Expression in Rats with Acute Lung Injury

doi:10.3969/j.issn.1671-2587.2016.03.002

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2016, Vol. 18 ›› Issue (3): 204-208.DOI: 10.3969/j.issn.1671-2587.2016.03.002

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The Influence of Rapamycin on NF-κB p65 and STAT-1 Expression in Rats with Acute Lung Injury

LI Li-Wei, LI Zhi-qiang

The Sixth People’Hospital of Shanghai Jiaotong University, Shanghai 200233

Received:2016-01-15 Online:2016-06-20 Published:2016-09-21

Abstract

Abstract: ObjectiveTo study the NF-κB p65 and STAT-1 expression in Sprague Dawley rats with transfusion-related acute lung injury or acute pancreatitis associated lung injury. MethodSixty SD rats were randomly divided into TRALI group, APALI group, control group, rapamycin treated TRALI group, rapamycin treated APALI group, and rapamycin treated control group, with 10 animals in each. Gene expression of NF-κB p65 and STAT-1 was detected by qRT-PCR. The method of 2-△△Ct was used to calculate the relative qualification of target genes. ResultThe expression of NF-κB p65 and STAT-1 in TRALI and APALI groups was found to be down-regulated（P<0.05）, and APALI group decreased more than TRALI group（P<0.01）. The gene expression of NF-κB p65 and STAT-1 in rapamycin traeted TRALI group decreased more than in TRALI group（P<0.01）, while rapamycin intervention APALI group did not decrease significantly compared to APALI group（P>0.05）. ConclusionRapamycin might deteriorate pathological process of the lung injury in TRALI, but have limited effects on APALI.

Key words: Acute, lung, injury, Transfusion, Pancreatitis, Gene, NF-κB, p65, STAT-1

CLC Number:

R563R392.12

LI Li-Wei, LI Zhi-qiang. The Influence of Rapamycin on NF-κB p65 and STAT-1 Expression in Rats with Acute Lung Injury[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2016, 18(3): 204-208.

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The Influence of Rapamycin on NF-κB p65 and STAT-1 Expression in Rats with Acute Lung Injury

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