Abstract: Objective To investigate the significance of nucleic acid test in judging the results of serological weak-reactive blood samples.Methods From October 1,2019 to June 30,2020 blood samples of patients before operation or transfusion were collected from 5 clinical medical institutions.The serological weak-reactive samples of HBV,HCV and HIV were detected by HBV DNA,HCV RNA,HIV-1/2 RNA test.Results Among the 5 782 samples,53 cases showed weak reaction,of which HBV positive in 24 cases,HCV positive in 25 cases and HIV positive in 4 cases.Further nucleic acid detection showed that 15 cases of HBV nucleic acid detection were reactive and 9 non-reactive;25 cases of HCV nucleic acid detection were non-reactive;4 cases of HIV nucleic acid detection were non-reactive.Conclusion The serological weak-reactiveis caused by high sensitivity of chemiluminescence method,which leads to a certain degree of false positive results.Nucleic acid detection canboth improvethe specificity and sensitivity,so as to ensure the accuracy of the results.

Key words: Hepatitis B virus, Hepatitis C virus, Human immunodeficiency virus, Serological detection, Nucleic acid detection