• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (4): 308-311.DOI: 10.3969/j.issn.1671-2587.2016.04.002

• 临床输血研究 • 上一篇    下一篇

去白细胞悬浮红细胞质量分析

  

  1. 523930 广东省东莞市中心血站(秦艳兰,莫海英,叶柱江,何子毅);中医院输血科(谭少华)
  • 出版日期:2016-08-20 发布日期:2016-08-26
  • 通讯作者: 何子毅(1974–),男,主任技师,硕士,主要从事输血安全与血液血型学研究,(Tel)0769-85152673。
  • 作者简介:秦艳兰(1967–),女,广西桂林人,副主任技师,主要从事血液质量检测和血液质量管理工作,(Tel)0769-85701386,13829169611。
  • 基金资助:
    本课题受东莞市科技局项目(No.201410515000851)资助

A Quality Analysis of Leukocytes Reduced Suspended Red Blood Cells

      

  1. Dongguan Blood Center , 523000
  • Online:2016-08-20 Published:2016-08-26

摘要: 目的 分析本中心制备的去白细胞悬浮红细胞的质量及其影响因素,为临床红细胞输注评估指标、合理输注红细胞剂量提供理论依据和参考方法。方法 收集100袋去白细胞悬浮红细胞质量检测结果, 分析其血容量、血红蛋白含量、红细胞压积和储存期末溶血率。结果   50袋1.5 U去白细胞悬浮红细胞血容量为(198±22)ml/袋,与理论值相比合格率为78%;血红蛋白含量(35.04±6.24)g,合格率92% ;红细胞压积0.52±0.06,合格率9 2%;白细胞残留量(2.15±0.80)×106个/袋,合格率9 6%;储存期末溶血率(0.1 3±0.12)%,合格率1 0 0%。5 0袋2 U去白细胞悬浮红细胞血容量(2 7 2±2 6)ml/袋,与理论值相比合格率为7 5%;血红蛋白含量(5 1 .2 3±6 .3 8) g,合格率9 6%;红细胞压积0.55±0.04,合格率1 0 0%;白细胞残留量(3.00±1.23)×106个/袋,合格率9 4%;储存期末溶血率(0.27±0.15)%,合格率100%。结论   本中心制备的去白细胞悬浮红细胞血液制品无论是1 .5 U还是2 U,其质量指标均符合2012版《血站技术操作规程》,能满足临床输血的质量要求。

关键词: 去白细胞悬浮红细胞, 血红蛋白含量, 储存期末溶血率

Abstract:   Objective To investigate the quality and the influence factors of red blood cells in additive solution with reduced leukocytes to provide theoretical basis and reference methods of assessment indicators and reasonable dose for clinical red blood cell transfusion. Methods The blood volume, hemoglobin hematocrit and the hemolytic rate were analyzed at the end of storage in 100 bags of red blood cells in additive solution with leukocytes reduced. Result In the
group of 1.5 U red blood cells in additive solution with leukocytes reduced in 5 0 bags, the average blood volume was (198±22 ) ml/bag, and the qualified rate, compared with the theoretical value, was 78%. The hemoglobin concentration was (35.04±6.24 ) g, and the qualified rate was 92%. The hematocrit was (0.52±0.06 ) and, the qualified rate was 92%. The residual quantification of leukocyte was (2.15±0.80 )×1 06/bag, and the qualified rate was 9 6%. The hemolytic rate at the end of storage was (0 .1 3±0 .1 2 )%, and the qualified rate was 1 0 0%. In the group of 2 .0 U red blood cells of 50 bags in additive solution with leukocytes reduced, the average blood volume was (272±26 ) ml/bag, and the qualified rate, compared with the theoretical value, was 75%. The hemoglobin concentration was (51.23±6.38 ) g, and the qualified rate was 96%. The hematocrit was (0.55±0.04 ), and the qualified rate was 100%. The residual quantification of leukocyte was (3.00±1.23 )×106/bag,and the qualified rate was 94%. The hemolytic rate at the end of storage was (0.27±0.15 )%, and the qualified rate is 100%. Conclusion The quality indicators of blood products in both 1.5 U and 2.0 U of red blood cells in additive solution with leukocytes reduced met the standard of 2012 edition of "Technical Operating Procedures in Blood Station".

Key words: Red blood cells reduced leukocytes, Hemoglobin Hemolysis rate