• 中国科学论文统计源期刊
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临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (5): 508-509.DOI: 10.3969/j.issn.1671-2587.2017.05.030

• 技术与方法 • 上一篇    下一篇

化学发光法检测人类免疫缺陷病毒抗体高值阴性样本的分析

靳丽丽, 王晓伟, 邵春燕, 骆群   

  1. 100071 北京市解放军三〇七医院输血科
  • 收稿日期:2017-03-10 出版日期:2017-10-20 发布日期:2017-10-26
  • 通讯作者: 骆群（1967-）,男,主要从事临床输血与研究,（E-mail）luoq66@aliyun.com。
  • 作者简介:靳丽丽（1988-）,女,检验技师,学士,主要从事传染病检测工作,（Tel）18141903126（E-mail）zhaoning2008061106@126.com。

Confirmatory Tests of Negative Samples Near the Critical Value in Human Immunodeficiency Virus Antibody Detection by Chemiluminescence Immunoassay

JIN Li-li, WANG Xiao-wei, SHAO Chun-yan, et al   

  1. Departmeng of Blood Transfusion Chinese PLA 307 Hospital,Beijing 100071
  • Received:2017-03-10 Online:2017-10-20 Published:2017-10-26

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摘要： 目的 分析化学发光法（chemiluminescence immunoassay,CLIA）检测人类免疫缺陷病毒（human immunodeficiency virus,HIV）抗体高值阴性样本是否有潜在危险性。方法 用CLIA试剂在临床标本中筛选出HIV抗体0.3<S/CO（absorbance signal of sample/cut off value）<0.9的血清样本,用酶联免疫吸附法（enzyme linked immunosorbent assay,ELISA）复查,将ELISA法阳性反应标本用蛋白印迹（Westernblot,WB）确证并分析结果。结果 用CLIA试剂从64 425份临床标本中筛查出CLIA检测HIV抗体0.3<S/CO<0.9的血清样本134例。其中ELISA试剂有阳性反应的标本2例,且此2例患者4周后复查均为阴性。结论 CLIA检测HIV抗体0.3<S/CO<0.9的样本可直接发放阴性结果,无潜在危险性。

关键词: 化学发光, 酶联免疫吸附法, 人类免疫缺陷病毒抗体, 蛋白印迹

Abstract: Objective To reveal the potential risk of the negative samples near the critical value in detection of human immunodeficiency virus antibody by chemiluminescence immunoassay. Method The samples were selected by chemiluminescence immunoassay（CLIA） for antibodies against HIV in which absorbance of the sample's cutoff value（S/CO）between 0.3 and 0.9,and checked by enzyme linked immunosorbent assay（ELISA）. Result One hundred and thirty-four samples over 0.3<S/CO<0.9 were screened from 64425 clinical samples by CLIA. Among them,2 cases were positive withr ELISA. The two positive cases,however,turned to be negative four weeks later. Conclusion Detection of CLIA HIV antibodies based on the threshold of 0.3<S/CO<0.9 will be able to ensure the safety in HIV diagnosis.

Key words: Chemiluminescence immunoassay, Enzyme linked immunosorbent assay, HIV antibody, Western blot

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