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临床输血与检验

临床输血与检验 ›› 2018, Vol. 20 ›› Issue (2): 150-153.DOI: 10.3969/j.issn.1671-2587.2018.02.013

• 临床检验 • 上一篇    下一篇

深圳地区急性上吸呼道感染住院患儿人偏肺病毒感染现状及基因分析*

刘爱玲, 刘淑燕, 刘军, 朱彩云, 郭龙华   

  1. 518109 广东省深圳市龙华区人民医院检验科（刘爱玲,刘淑燕,朱彩云,郭龙华）; 广东医科大学病原生物学实验室（刘军）
  • 收稿日期:2017-11-18 出版日期:2018-04-20 发布日期:2018-04-25
  • 作者简介:刘爱玲（1983-）,女,湖南人,主管技师,硕士,主要从事微生物和分子生物学检验工作,（Tel）18098942620（E-mail）liuailing1983@163.com。
  • 基金资助:
    *本课题受广东医科大学校院联合科研基金（No.L2016008）资助

Gene Analysis of Pulmonary Virus in the Hospitalized Children with Acute Upper Respiratory Trace Infection and its Present Situation

LIU Ai-ling, LIU Shu-yan, LIU Jun, et al   

  1. Clinical Laboratory of Shenzhen Longhua District People's Hospital,Guangdong Province 518109
  • Received:2017-11-18 Online:2018-04-20 Published:2018-04-25

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摘要： 目的 了解深圳地区急性上呼吸道感染（acute upper respiratory tract infection,AURTI）住院患儿人偏肺病毒（human metapneumovirus,hMPV）的感染现状及hMPV优势亚型的流行情况。 方法 收集2015年6月~2016年6月在深圳市龙华区人民医院和福田人民医院因ALRTI住院的患儿鼻咽吸取物或咽拭子标本1 720份,同时收集同期健康儿童呼吸道标本60份,采用Qiagen One-step RT-PCR法对标本中的hMPV进行筛检,并随机选取6例hMPV阳性标本采用CEQ8800测序仪对扩增产物进行正反测序,采用BLAST 软件将测序所得的基因序列与GenBank中的代表株进行序列比对分析。 结果 1 720例AURTI住院患儿的鼻咽吸取物或咽拭子标本共检出 hMPV阳性标本362例,阳性率为21.05%（362/1 720）,其中男童阳性率为10.06%（173/1 720）,女童阳性率为10.99%（189/1 720）,不同性别患儿阳性率的差异无统计学意义（χ2=0.917,P>0.05）,而健康儿童hMPV全为阴性;<1岁组AURTI住院患儿hMPV感染阳性率为31.46%,明显高于其他组,差异有统计学意义（χ2=6.105~11.973,P（0.05~0.01）;1~3月AURTI住院患儿感染hMPV阳性率最高为49.74%,明显高于其他月份,差异有统计学意义（χ2=7.927~15.038,P<0.05~0.01）;6株hMPV阳性毒株的病毒核苷酸与代表株同源性为91.0％~100.0%;6株hMPV阳性毒株分别属于A2b和B1及B2亚型。 结论 hMPV是引起深圳地区AURTI住院患儿感染的主要病原之一,好发于冬春季节和1岁以下儿童,且同时存在A和B两个基因型流行。

关键词: 急性上呼吸道感染, 患儿, 人偏肺病毒, 现状, 基因分析

Abstract: Objective To understand acute upper respiratory trace infections （acute upper respiratory tract infection,AURTI） people hospitalized children with partial pulmonary virus（ human metapneumovirus,hMPV） infection status and the prevalence of hMPV advantage subtypes in Shenzhen area. Methods From June 2015 to June 2016 in shenzhen longhua district people's hospital and Hutian people's hospital hospitalized Children with AURTI nasopharyngeal drain or swallow test specimens 1 720,at the same time respiratory specimens from 60 healthy children of the same period were collected,Qiagen One - step RT-PCR method for screening of hMPV in the sample was employed,and amplification products randomly selected from 6 cases of hMPV positive specimens were sequenced by CEQ8800 sequencer through forward and reverse primers,BLAST software was used to sequence alignment analysis for sequencing of gene sequences with GenBank representative strains . Results Nasopharyngeal hMPV positive specimens of 362 cases were detected from 1 720 cases of hospitalized children with AURTI drains content or swallow test specimen,positive rate was 21.05% （362/1 720）,the boy's positive rate was 10.06% （173/1 720）,the girl's positive rate was 10.99% （189/1 720）,there was no statistically significant difference between male and female children with positive rate （χ2=0.917,P>0.05）,and healthy children hMPV were all negative; <1 year old group AURTI hospitalized with an hMPV infection rate was 31.46%,significantly higher than the other group,and the difference was statistically significant （χ2=6.105~11.973,P<0.05~0.01）;1~3 month AURTI hospitalized children with hMPV infection positive rate was 49.74%,significantly higher than the other month,difference was statistically significant between （χ2=7.927~15.038,P<0.05~0.01）; The virus nucleotide of the six hMPV positive strains was 91.0%~100.0%. 6 strains of hMPV positive strains belong to the A2b and B1 and B2 subtype respectively. Conclusionh MPV is one of the main pathogens of infection in children with AURTI in the hospital in Shenzhen area,occurs in winter and spring seasons and children under the age of 1,A and B are two prevalent genotypes.

Key words: Acute upper respiratory tract infection, Children, Human pneumovirus, Status, Genetic analysis

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