支气管哮喘患儿白介素、TNF-α与肺功能及免疫功能的关系研究

doi:10.3969/j.issn.1671-2587.2020.06.019

临床输血与检验 ›› 2020, Vol. 22 ›› Issue (6): 638-642.DOI: 10.3969/j.issn.1671-2587.2020.06.019

支气管哮喘患儿白介素、TNF-α与肺功能及免疫功能的关系研究

何雯, 毕良学, 王宁玲

230000 安徽省儿童医院,安徽医科大学附属省儿童医院,复旦大学附属儿科医院安徽医院(何雯,毕良学),安徽医科大学第二附属医院(王宁玲)

收稿日期:2019-03-27 出版日期:2020-12-20 发布日期:2021-01-18
通讯作者: 王宁玲,女,教授,主任医师,主要从事儿童血液病方面研究,(E-mail)zwnltt@126.com。
作者简介:何雯(1986-),女,安徽池州人,本科,主治医师,主要从事儿内科方面研究,(Tel)13965130961(E-mail)58618913@qq.com。

Study on Relationship between Interleukin,TNF-αand Lung Function and Immune Function in Children with Bronchial Asthma

HE Wen, BI Liang-xue, WANG Ning-ling

Department of Respiratory Medicine,Anhui Children's Hospital,Hefei 230000

Received:2019-03-27 Online:2020-12-20 Published:2021-01-18

摘要/Abstract

摘要： 目的研究支气管哮喘患儿白介素和TNF-α与肺功能、免疫功能的关系。方法将178例支气管哮喘患儿分为发作组(急性发作期,107例)及缓解组(缓解期,71例),另取同期体检健康者83例作为对照组。均检测白介素[白介素-2(IL-2)、白介素-4(IL-4)、白介素-6(IL-6)]、肿瘤坏死因子-α(TNF-α)、肺功能[用力肺活量(FVC)、第一秒用力呼气容积(FEV₁)、FEV₁/FVC、呼气峰值流速(PEF)]及免疫功能[T淋巴细胞亚群(CD4⁺、CD8⁺、CD4⁺/CD8⁺)],并采用Pearson相关性分析法分析血清炎性因子水平与肺功能、免疫功能的关系。结果血清IL-2、IL-4、IL-6及TNF-α水平以发作组最高,缓解组次之,对照组最低,差异显著(P<0.05);FVC、FEV₁、FEV₁/FVC、PEF、CD4⁺、CD4⁺/CD8⁺水平以发作组最低,缓解组次之,对照组最高,差异显著(P<0.05);CD8⁺水平在各组间无明显差异(P>0.05);经Pearson相关性分析,发作组和缓解组血清IL-2、IL-4、IL-6、TNF-α水平与FVC、FEV₁、FEV₁/FVC、PEF、CD4⁺、CD4⁺/CD8⁺均呈负相关(P<0.05),与CD8⁺无相关性(P>0.05)。结论支气管哮喘患儿白介素和TNF-α显著升高,肺功能及免疫功能均明显降低,且白介素和TNF-α与肺功能及免疫功能呈负相关性,临床中可将以上指标作为评估支气管哮喘患儿治疗效果的重要依据。

关键词: 支气管哮喘, 患儿, 白介素, 肺功能, 免疫功能, 相关性

Abstract: Objective To study the relationship between interleukin,TNF-αand lung function and immune function in children with bronchial asthma.Methods 178 children with bronchial asthma were divided into acute attack group(n=107) and remission group(n=71),and 83 healthy persons at the same period were enrolled in control group.The serum levels of interleukin [interleukin-2(IL-2),interleukin-4(IL-4)and interleukin-6(IL-6)],tumor necrosis factor-α(TNF-α),lung function related indicators [forced vital capacity(FVC),forced expiratory volume in the first second(FEV₁),FEV1/FVC and peak expiratory flow(PEF)] and immune function related indicators [T lymphocyte subsets(CD4⁺,CD8⁺andCD4⁺/CD8⁺)] were measured.Pearson correlation analysis was used to analyze the relationship between serum inflammatory factors and pulmonary function and immune function.Results The serum levels of IL-2,IL-4,IL-6 and TNF-αwere significantly increased in attack group(P<0.05).The levels of FVC,FEV1,FEV₁/FVC,PEF,CD4⁺and CD4⁺/CD8⁺ were significantly decreased in attack group(P<0.05).There was no significant difference in the levels of CD8⁺ among groups(P>0.05).Pearson correlation analysis showed that serum levels of IL-2,IL-4,IL-6 and TNF-αwere negatively correlated with FVC,FEV₁,FEV₁/FVC,PEF,CD4⁺,CD4⁺/CD8⁺ in attack group and remission group(P<0.05),but no with CD8⁺(P>0.05).Conclusion The levels of interleukin and TNF-αin children with bronchial asthma are significantly increased,while lung function and immune function are significantly reduced.Moreover,interleukin and TNF-αhave a negative correlation with lung function and immune function.The above indicators can be used to evaluate the therapeutic effect of bronchial asthma in children.

Key words: Bronchial asthma, Children Interleukin, Lung function, Immune function, Correlation

中图分类号:

R562.25

何雯, 毕良学, 王宁玲. 支气管哮喘患儿白介素、TNF-α与肺功能及免疫功能的关系研究[J]. 临床输血与检验, 2020, 22(6): 638-642.

HE Wen, BI Liang-xue, WANG Ning-ling. Study on Relationship between Interleukin,TNF-αand Lung Function and Immune Function in Children with Bronchial Asthma[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(6): 638-642.

参考文献

[1] ERKMAN J,VAYNBLAT A,THOMAS K,et al.Airway and esophageal eosinophils in children with severe uncontrolled asthma[J].Pediatr Pulmonol,2018,53(12):1598-1603.
[2] HASSAN S H,GHANI R,SARWAR M.Molecular basi of superoxide dismutase alterations in Spirometry proven bronchial asthma[J].JPMA J Pak Med Assoc,2017,67(9):1393-1397.
[3] LAZOVA S,PERENOVSKA P,MITEVA D,et al.Measuring the bronchial reversibility in asthmatic children-clinical value of different indices[C]//Paediatric Asthma and Allergy.European Respiratory Society,2017.
[4] NUNES C F,NOGUEIRA J S,VIANNA P H O,et al.Probiotic treatment during neonatal age provides optimal protection against experimental asthma through the modulation of microbiota and T cells[J].Int Immunol,2018,30(4):155-169.
[5] 陈育智.儿童支气管哮喘的诊断及治疗[M].北京.人民卫生出版社,2010:17-18.
[6] RUSSELL R,SINGAPURI A,BERAIR R,et al.Clinical and histological effects of Bronchial Thermoplasty in severe asthma[C]//Interventional Pulmonology.European Respiratory Society,2017.
[7] Alcázar-Navarrete B,Castellano Miñán F,Santiago Díaz P,et al.Alveolar and bronchial nitric oxide in chronic obstructive pulmonary disease and asthma-COPD overlap[J].Arch De Bronconeumología Engl Ed,2018,54(8):414-419.
[8] KIM J H,CHANG H S,SHIN S W,et al.Lung function trajectory types in never-smoking adults with asthma:clinical features and inflammatory patterns[J].Allergy Asthma Immunol Res,2018,10(6):614.
[9] 曾静,靳蓉,陈敏,等.血清IL-8、IgE、TNF-α和外周血 EOS 水平在儿童哮喘中的表达及意义[J].疑难病杂志,2015,14(6):604-606.
[10] 阎敏娜,项保利,张素贞,等.支气管哮喘患儿IL-17及IL-35水平变化及意义[J].临床儿科杂志,2018,36(4):268-271.
[11] OZKARS M Y,KESKIN O,TOKUR M,et al.Comparing the effects of fluticasone,anti-IgE and anti-TNF treatments in a chronic asthma model[J].Allergol et Immunopathol,2018,46(3):226-234.
[12] RAY A,KOLLS J K.Neutrophilic inflammation in asthma and association with disease severity[J].Trends Immunol,2017,38(12):942-954.
[13] 王兰英,吴慧莲,唐江利.潮气呼吸肺功能检测在婴幼儿支气管哮喘中的应用[J].海南医学,2017,28(19):3234-3236.
[14] 焉石.自拟祛风平喘汤对风痰闭阻型支气管哮喘患者细胞免疫功能的影响[J].中医药学报,2016,44(1):91-93.
[15] TUMES D J,PAPADOPOULOS M,ENDO Y,et al.Epigenetic regulation of T-helper cell differentiation,memory,and plasticity in allergic asthma[J].Immunol Rev,2017,278(1):8-19.
[16] 雷艳青,陈波.六味地黄丸联合西药对支气管哮喘患者免疫功能的影响[J].陕西中医,2017,38(4):461-462.
[17] Elieh Ali Komi D,Bjermer L.Mast cell-mediated orchestration of the immune responses in human allergic asthma:current insights[J].Clin Rev Allergy Immunol,2019,56(2):234-247.
[18] QIU Z W,ZHOU J S,LIU F,et al.Deletion of Shp2 in bronchial epithelial cells impairs IL-25 production in vitro,but has minor influence on asthmatic inflammation in vivo[J].PLoS One,2017,12(5):e0177334.
[19] 周戈会,邹帮铎.血清IL-6、IL-8、TNF-α水平变化与支气管哮喘患儿免疫功能的相关性研究[J].临床医药文献电子杂志,2016,3(20):4013.

支气管哮喘患儿白介素、TNF-α与肺功能及免疫功能的关系研究

Study on Relationship between Interleukin,TNF-αand Lung Function and Immune Function in Children with Bronchial Asthma

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