• 中国科学论文统计源期刊
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临床输血与检验

临床输血与检验 ›› 2020, Vol. 22 ›› Issue (5): 477-480.DOI: 10.3969/j.issn.1671-2587.2020.05.008

• 临床输血 • 上一篇    下一篇

二次重度离心制备法对冷沉淀凝血因子质量的影响*

余凤秀, 李津杞, 朱燕霞, 郭菲, 周载鑫, 顾海慧, 钱宝华   

  1. 200433 上海长海医院输血科
  • 收稿日期:2019-11-20 出版日期:2020-10-20 发布日期:2020-10-16
  • 通讯作者: 钱宝华,男,江苏海安人,主任医师,教授,博士研究生导师,主要从事临床输血研究工作,（Tel） 021-31162121（E-mail）qianbh1963@163.com。
  • 作者简介:余凤秀（1976-）,女,安徽安庆人,护师,主要从事临床输血相关工作,（Tel）021-31162126（E-mail） y13801736333@163.com。
  • 基金资助:
    基金项目*本课题受国家自然科学基金面上项目（No.81570185、No.81970165）资助

Effect on the Quality of Cryoprecipitation from Fresh Frozen Plasma by High Speed Centrifugation Twice

YU Feng-xiu, LI Jin-qi, ZHU Yan-xia, et al   

  1. Department of Transfusion Medicine, Changhai Hospital, Navy Military Medical University, Shanghai 200433
  • Received:2019-11-20 Online:2020-10-20 Published:2020-10-16

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摘要： 目的 探讨一次或二次重度离心制备新鲜冰冻血浆（冷沉淀凝血因子原料浆）对冷沉淀凝血因子质量的影响。 方法 检测冷沉淀凝血因子中游离血红蛋白含量、Ⅷ因子和纤维蛋白原含量。全血40单位（每单位200 mL）分成对照组、实验组,每组n=20。按国家标准采集、离心和分离,再将实验组新鲜冰冻血浆（冷沉淀凝血因子原料浆）进行第二次离心和分离,同时放入-80℃深低温冰箱速冻,储存时间为14天。14天后经过离心法制备成冷沉淀凝血因子,分别进行残留血红蛋白、Ⅷ因子和纤维蛋白原含量的检测。 结果 实验组残留血红蛋白含量（17.64±5.60）mg/L,显著低于对照组（25.75±9.78）mg/L（t=0.035,P<0.05）;实验组Ⅷ因子含量（53.26±6.08）IU/单位,合格率100%高于对照组（47.34±9.14）IU/单位、合格率80%（t=0.041,P<0.05）;实验组纤维蛋白原含量（162±29.01）mg/单位,高于对照组（147.28±43.39）mg/单位,两组合格率均100%（t=0.38,P>0.05）。 结论 二次重度离心新鲜冰冻血浆（冷沉淀凝血因子原料浆）能使残留红细胞更少,使残留游离血红蛋白更低,可以提高冷沉淀凝血因子中Ⅷ因子的含量,从而提高冷沉淀凝血因子制备质量。

关键词: 新鲜冰冻血浆, 冷沉淀凝血因子, 游离血红蛋白, Ⅷ因子, 纤维蛋白原

Abstract: Objective To study the effect on the quality of cryoprecipitation from fresh frozen plasma which was centrifugated with high speed once or twice. We detected the content of free hemoglobin, factor Ⅷ and fibrinogen in cryoprecipitate. Methods 40 units whole blood（200 mL per-unit）were divided into two groups. The fresh frozen plasma in control group was centrifugated for once, and that in experimental group was centrifugated twice. Then the following separations were in accordance with national standards. We got the cryoprecipitate of two groups 14 days later, then detected the content of free hemoglobin, factor Ⅷ and fibrinogen in cryoprecipitate. Results Free hemoglobin in the experimental group (17.64±5.60) mg/L is lower than that in the control group (25.75±9.78) mg/L, (P<0.05); and in the experimental group, the content of factor Ⅷ is (53.26±6.08) IU/unit, which is higher than that in the control group （47.34±9.14）IU/ unit（P<0.05）; and the content of fibrinogen is（162±29.01）mg/ unit, which is higher than that in the control group (147.28±43.39) mg/ unit, （P>0.05）. Conclusion By additional high speed centrifugation, more residual red blood cells can be removed from fresh frozen plasma, which may improve the quality of the cryoprecipitation, and increase the content of factor Ⅷ and fibrinogen.

Key words: Fresh frozen plasma, Cryoprecipitate, Free hemoglobin, Factor &#x02167, Fibrinogen

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