• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (3): 324-327.DOI: 10.3969/j.issn.1671-2587.2021.03.008

• 临床输血 • 上一篇    下一篇

不同抗原格局抗筛细胞联合应用的意义探讨*

闫烨, 张勤, 王丽娜, 荆浩, 吕毅, 兰炯采   

  1. 450018 郑州颐和医院(闫烨,张勤,王丽娜,荆浩,吕毅); 南方医科大学南方医院(兰炯采)
  • 收稿日期:2020-05-29 出版日期:2021-06-20 发布日期:2021-06-22
  • 通讯作者: 吕毅,女,主任技师,主要从事临床输血检验方面研究,(E-mail)13598026289@163.com。
  • 作者简介:闫烨(1979-),女,河南郑州人,主管技师,硕士,主要从事临床输血检验方面研究,(Tel)13783622960。
  • 基金资助:
    *本课题受河南省科技攻关项目(No.201602349)资助

Combined Application of Screening Cells with Two Different Antigen Patterns

YAN Ye, ZHANG Qin, WANG Li-na, et al   

  1. Zhengzhou Yihe Hospital, Zhengzhou Henan 450018
  • Received:2020-05-29 Online:2021-06-20 Published:2021-06-22

摘要: 目的 探讨不同抗原格局抗筛细胞联合应用的意义,避免不规则抗体漏检。方法 选取1 200例拟输血患者血浆标本,先用抗人球蛋白检测卡进行不抗规则体筛查,实验结果阳性标本用谱细胞鉴定抗体特异性;实验结果阴性标本分别再用不同实验方法和不同筛选细胞进行检测。结果 不规则抗体筛查总阳性率为0.75%(9/1 200),其中使用单一试剂检出阳性率为0.58%(7/1 200),对其余抗筛结果阴性标本选用两种不同抗原格局筛选细胞检出抗体阳性2例,漏检率为0.17%(2/1 193)。结论 应用木瓜酶法可以提高抗体检出率,不同抗原格局抗筛细胞联合应用可以有效预防不规则抗体漏检,降低输血不良反应的发生。

关键词: 不规则抗体筛查, 抗体鉴定, 木瓜酶

Abstract: Objective To explore the significance of combined application of screening cells with different antigen patterns to avoid missed detection of irregular antibodies. Methods Plasma specimens of 1 200 patients undergoing blood transfusion were selected. Gel test was used for irregular antibodies. Positive specimen was further evaluated for identification of specific alloantibody with cell panel. Negative specimen was tested by different methods and screening cells. Results The total positive rate of irregular antibodies was 0.75% (9/1 200), of which the positive rate tested by a single reagent was 0.58% (7/1 200). Two different antigen patterns were used to screen the remaining negative samples, two samples were positive and the missed detection rate was 0.17% (2 /1 193). Conclusion The use of papain enzymatic method can increase the positive rate of alloantibodies detection. The combined application of screening cells with different antigen patterns can effectively prevent the missed detection of irregular antibodies and reduce the occurrence of transfusion reactions.

Key words: Irregular antibody screening, Antibodies identification, Papain

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