云南地区育龄人群血小板抗原基因多态性初步研究*

doi:10.3969/j.issn.1671-2587.2021.06.014

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (6): 737-741.DOI: 10.3969/j.issn.1671-2587.2021.06.014

云南地区育龄人群血小板抗原基因多态性初步研究^*

罗梓瑜, 董伟群

650032 云南,昆明医科大学第一附属医院

收稿日期:2021-09-05 出版日期:2021-12-20 发布日期:2021-12-21
通讯作者: 董伟群,女,主任医师,硕士,主要从事临床输血方面研究,（E-mail）1552454825@qq.com。
作者简介:罗梓瑜（1993-）,女,云南昆明人,技师,本科,主要从事临床输血方面研究,（E-mail）446792729@qq.com。
基金资助:
*本课题受云南省科技厅昆医联合专项（No.2018FE001（-217））资助

Preliminary Analysis of Human Platelet Antigen Genetic Polymorphism in People of Childbearing Age from Yunnan Province

LUO Zi-yu, DONG Wei-qun

First Affiliated Hospital of Kunming Medical University 650032

Received:2021-09-05 Online:2021-12-20 Published:2021-12-21

摘要/Abstract

摘要： 目的分析云南地区育龄人群血小板特异性抗原HPA1-5系统基因多态性,为地区内FNAIT等疾病的诊断治疗提供理论依据。方法采用聚合酶链-序列特异性引物技术（PCR-SSP）对云南省内不同民族的576名育龄期健康志愿者HPA1-5系统进行基因分型,分别计算其基因频率、基因型频率,进行分析比较。对本地区630名来自产科和生殖遗传科育龄妇女进行血小板抗体筛查,根据不同民族基因频率分析抗体检测结果。结果 5个HPA系统中,景颇族HPA-4基因型均为aa,未检测到b等位基因;其余民族及其他HPA系统同时检测到a和b等位基因,除HPA-3系统外均以aa基因型为主。阿昌族HPA-1系统基因频率与其他民族相比有差异,傣族组的HPA-2系统的基因频率与其他组的差异具有统计学意义,汉族组HPA-5系统对偶抗原不配合概率显著低于其他民族组;血小板抗体筛查结果显示,阳性病例血小板抗体很有可能为抗-HPA3系统的抗体。结论云南地区不同民族HPA1-5系统基因频率的分布具有各自的特点,对疾病的预防诊断治疗时应考虑民族因素的影响。

关键词: 血小板抗原, 基因多态性, 少数民族

Abstract: Objective To investigate the genetic polymorphism of HPA1-5 alleles in people of childbearing age from Yunnan Province and to provide valuable data for diagnosis and treatment of FNAIT and other diseases. Methods PCR-SSP was adopted to genotype the HPA1-5 system in 576 cases from different nationalities, Gene frequency and noncooperative rate of the population in Yunnan were calculated and were compared with each other. Platelet antibody screening was carried out among 630 women of childbearing age in Yunnan, andresults were analyzed according to gene frequency of different nationalities. Results None of HPA-b was found in Jingpo population samples and all the HPA-4 genotypes of Jingpo were aa. aa homozygosity was predominant except HPA-3. HPA-1a frequency of Achang was significantly different from that other populations in same study. HPA-2 frequency of Jingpo was significantly different from that other populations in our study.The MP of HPA-5 system in Han group was significantly lower than that in other ethnic groups. Platelet antibody-screening results showed that antibodies against-HPA3 system were the most in positive cases. Conclusion The distribution of HPA1-5 gene frequency in different ethnic groups in Yunnan has its own characteristics, and the influence of ethnic factors should be considered in the prevention, diagnosis and treatment of obstetric diseases.

Key words: Human platelet antigens, Gene polymorphism, Ethnic minorities

中图分类号:

R394

罗梓瑜, 董伟群. 云南地区育龄人群血小板抗原基因多态性初步研究^*[J]. 临床输血与检验, 2021, 23(6): 737-741.

LUO Zi-yu, DONG Wei-qun. Preliminary Analysis of Human Platelet Antigen Genetic Polymorphism in People of Childbearing Age from Yunnan Province[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(6): 737-741.

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云南地区育龄人群血小板抗原基因多态性初步研究^*

Preliminary Analysis of Human Platelet Antigen Genetic Polymorphism in People of Childbearing Age from Yunnan Province

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