改良脐带血分离方法的质控样管复苏回顾研究

doi:10.3969/j.issn.1671-2587.2023.02.016

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (2): 239-242.DOI: 10.3969/j.issn.1671-2587.2023.02.016

改良脐带血分离方法的质控样管复苏回顾研究

吴春燕, 陈阳, 李星洁, 王自波, 陈强, 赖真阳

610000 四川成都,四川省脐带血造血干细胞库,四川新生命干细胞科技股份有限公司

收稿日期:2022-11-18 发布日期:2023-04-25
通讯作者: 赖真阳,副研究员,主要从事干细胞方面的研究。（E-mail）lai.zhenyang@foxmail.com。
作者简介:吴春燕,主要从事脐带血制备冻存方面的研究,（E-mail）303506684@qq.com。

A Retrospective Study on a Modified Cord Blood Separation Method Using QC Vial Thawing Data

WU Chun-yan, CHEN Yang, LI Xing-jie, et al

Sichuan Cord Blood Bank 610000

Received:2022-11-18 Published:2023-04-25

摘要/Abstract

摘要： 目的对比改良脐带血分离方法（改良方法）与传统富浆法（传统方法）对于脐带血造血干细胞质量的影响。方法根据不同体积段改良传统脐带血分离方法,对近年来复苏的脐带血质控样管进行回顾研究,对比方法改良前后一年,两种制备方法获得产品的各项质量参数。结果使用改良方法后,分离后体积由传统方法的（25.68±2.03）mL下降至（22.53±4.24）mL（P<0.05）。红细胞（RBC）去除率由（75.87±11.84）%上升至（82.94±8.68）%（P<0.001）。复苏的质控样管在总有核细胞（TNC）回收、CD34⁺细胞回收、CD34⁺细胞活率等方面两组间无统计学差异。集落形成能力检测中,改良方法组的粒单核细胞系集落形成单位（CFU-GM）回收率为（60.00±32.52）%,较传统方法组的（46.97±28.12）%有提高,且差异具有统计学意义（P<0.05）。结论改良脐带血分离方法提高了RBC去除率,减小了冻存体积,复苏脐带血干细胞具有更好的CFU-GM形成能力。

关键词: 脐带血, 改良脐带血分离法, 质控样管复苏

Abstract: Objective To compare the impact on stem cell quality between using modified cord blood separation method (modified method) and using traditional cord blood separation method (traditional method). Methods We developed a modified method, in which different centrifugation parameters were used according to the samples' volume. We collected QC vials' thawing data, which were processed within one year before and after the modification. Test results were compared between these two methods. Results In modified method group, volume after separation decreased from (25.68±2.03) mL to (22.53±4.24) mL (P<0.05). Red blood cells (RBC) removal rate rose from (75.87±11.84)% to (82.94±8.68)% (P<0.001). No statistic difference was observed in total nucleated cells (TNC) recovery, CD34⁺ cell recovery and CD34⁺cell viability after thawing. In colony formation test, colony forming unit-granulocyte monocyte (CFU-GM) recovery were (60.00±32.52)% and (46.97±28.12)% in modified method group and traditional method group, respectively. The difference was statistically significant (P<0.05). Conclusion sThe modified cord blood separation method improved RBC removal rate and freezing product volume. After freezing, the stem cell showed higher potency in CFU-GM formation.

Key words: Cord blood, Modified cord blood separation method, QC vial thawing

中图分类号:

R-331

吴春燕, 陈阳, 李星洁, 王自波, 陈强, 赖真阳. 改良脐带血分离方法的质控样管复苏回顾研究[J]. 临床输血与检验, 2023, 25(2): 239-242.

WU Chun-yan, CHEN Yang, LI Xing-jie, et al. A Retrospective Study on a Modified Cord Blood Separation Method Using QC Vial Thawing Data[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(2): 239-242.

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改良脐带血分离方法的质控样管复苏回顾研究

A Retrospective Study on a Modified Cord Blood Separation Method Using QC Vial Thawing Data

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