NLRP3炎性小体对THP-1细胞吞噬老化红细胞后亚类分化的调控*

doi:10.3969/j.issn.1671-2587.2024.04.005

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (4): 457-462.DOI: 10.3969/j.issn.1671-2587.2024.04.005

NLRP3炎性小体对THP-1细胞吞噬老化红细胞后亚类分化的调控^*

李勤, 赵俸涌, 张嘉敏, 杨颖, 郭忠慧, 王晨, 杨启修, 朱自严

上海市血液中心,上海 200051

收稿日期:2024-06-27 出版日期:2024-08-20 发布日期:2024-09-23
通讯作者: 朱自严,主要从事免疫血液学研究,（E-mail）zhuziyan@sbc.org.cn。
作者简介:李勤,主要从事免疫血液学研究,（E-mail）liqin@sbc.org.cn。
基金资助:
*本课题受上海市科技计划项目（No.19ZR1450300）资助

Modulation of NLRP3 Inflammasome Activity in Subtype Differentiation of THP-1 Cells after Phagocytosis of Aged Erythrocyte

LI Qin, ZHAO Fengyong, ZHANG Jiamin, YANG Ying, GUO Zhonghui, WANG Chen, YANG Qixiu, ZHU Ziyan

Shanghai Blood Center, Shanghai 200051

Received:2024-06-27 Online:2024-08-20 Published:2024-09-23

摘要/Abstract

摘要： 目的明确NLRP3炎性小体是否可调节巨噬细胞对红细胞的吞噬能力,调控巨噬细胞的亚类分化。方法以NLRP3低表达的THP-1细胞（ID3 THP-1）,转空载体的THP-1细胞（shNC THP-1）及野生型THP-1细胞模拟巨噬细胞,与未处理红细胞、水浴老化红细胞、IgG致敏红细胞分别孵育。流式细胞术检测THP-1细胞对不同红细胞的吞噬率;流式细胞术检测巨噬细胞M1亚类指标CD16和CD86,M2亚类指标CD163和CD206在THP-1细胞上的表达。结果 NLRP3低表达的ID3 THP-1对红细胞的吞噬能力明显下调。老化红细胞具有诱导THP-1向M1亚类分化,抑制THP-1向M2亚类分化的能力。当THP-1的NLRP3炎性小体表达下降时,吞噬红细胞后,其向M1亚类分化的能力下调,而向M2亚类分化的能力增强。结论 NLRP3炎性小体可作为一个靶向调节位点,调控巨噬细胞对红细胞吞噬,及其自身的亚类分化。

关键词: 巨噬细胞, 老化红细胞, 吞噬, 巨噬细胞极化

Abstract: Objectives To clarify whether NLRP3 inflammasome can modulate macrophage phagocytic capacity towards red blood cells (RBCs) and regulate macrophage subtype polarization. Methods THP-1 cells with low expression of NLRP3 (ID3 THP-1), cells transfected with an empty vector (shNC THP-1), and wild-type THP-1 cells were used as macrophage models. These cells were incubated with untreated RBCs, water bath-aged RBCs, and IgG-opsonized RBCs, respectively. Flow cytometry was used to detect the phagocytic rate of THP-1 cells to different RBC types and to measure the expression of M1 subtype markers (CD16 and CD86), and M2 subtype markers (CD163 and CD206) on THP-1 cells. Results ID3 THP-1 with reduced NLRP3 expression exhibited significantly downregulated phagocytic capacity towards RBCs. Aged RBCs induced the differentiation of THP-1 cells into the M1 subclass while inhibiting their differentiation into the M2 subclass. Decreased expression of the NLRP3 inflammasome in THP-1 cells led to a downregulation of their ability to differentiate into the M1 subclass following RBC phagocytosis, accompanied by their enhanced capacity to differentiate into the M2 subclass. Conclusion NLRP3 inflammasome can serve as a pivotal regulatory target, governing macrophage phagocytosis of RBCs and their subsequent subclass differentiation.

Key words: Macrophage, Aged RBCs, Phagocytosis, Macrophage polarization

中图分类号:

R446

李勤, 赵俸涌, 张嘉敏, 杨颖, 郭忠慧, 王晨, 杨启修, 朱自严. NLRP3炎性小体对THP-1细胞吞噬老化红细胞后亚类分化的调控^*[J]. 临床输血与检验, 2024, 26(4): 457-462.

LI Qin, ZHAO Fengyong, ZHANG Jiamin, YANG Ying, GUO Zhonghui, WANG Chen, YANG Qixiu, ZHU Ziyan. Modulation of NLRP3 Inflammasome Activity in Subtype Differentiation of THP-1 Cells after Phagocytosis of Aged Erythrocyte[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(4): 457-462.

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NLRP3炎性小体对THP-1细胞吞噬老化红细胞后亚类分化的调控^*

Modulation of NLRP3 Inflammasome Activity in Subtype Differentiation of THP-1 Cells after Phagocytosis of Aged Erythrocyte

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