氢气抑制单采血小板活化和凋亡的作用研究*

doi:10.3969/j.issn.1671-2587.2025.06.005

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (6): 775-782.DOI: 10.3969/j.issn.1671-2587.2025.06.005

氢气抑制单采血小板活化和凋亡的作用研究^*

李津杞¹, 秦爱华¹, 任瑜菲², 唐河山¹, 陈翠敏², 钱宝华¹, 臧艳¹

¹中国人民解放军海军军医大学第一附属医院输血科,上海 200433;
²中国人民解放军海军军医大学第一附属医院临床研究中心,上海 200433

收稿日期:2025-03-25 出版日期:2025-12-20 发布日期:2025-12-24
通讯作者: 臧艳,主要从事输血科质量控制方面研究,E-mail：13817145306@163.com。共同通信作者：钱宝华,主要从事合理输血方面研究,E-mail：qianbh1963@163.com。
作者简介:李津杞,主要从事血液保存和质量控制方面研究,E-mail：ljq_421@163.com。并列第一作者：秦爱华,主要从事输血治疗方面研究,E-mail：qinaihua729@126.com。
基金资助:
^*本课题受海军军医大学第一附属医院“234学科攀峰计划”（No.2020YXK036）资助

Inhibitory Effects of Hydrogen on Activation and Apoptosis in Stored Apheresis Platelets

LI Jinqi¹, QIN Aihua¹, REN Yufei², TANG Heshan¹, CHEN Cuimin², QIAN Baohua¹, ZANG Yan¹

¹Department of Transfusion Medicine, First Affiliated Hospital of Naval Medical University, Shanghai 200433;
²Clinical Research Center of the First Affiliated Hospital of Naval Medical University, Shanghai 200433

Received:2025-03-25 Online:2025-12-20 Published:2025-12-24

摘要/Abstract

摘要： 目的探索氢气（hydrogen, H₂）抑制单采血小板活化和凋亡的作用研究。方法 10份单采血小板,每份平均装入16小袋中,随机分为H₂组和对照组,H₂组加入H₂至饱和,并每天补充H₂。（22±2）℃振荡保存8天,每天检测血小板计数,流式检测CD62P、PAC-1及凋亡的百分比,Western blotting检测Caspase-3、Caspase-9、Bax、Bcl-2蛋白的表达水平。结果血小板计数在保存期间（1~8天）的差异均无统计学意义。但流式实验的凋亡指标从第5天开始,H₂组的比例比对照组低（20.29±4.32 vs 33.06±8.01,P<0.001）;活化指标CD62P从第6天开始,H₂组比对照组低（24.94±5.53 vs 47.14 ±10.41,P<0.001）,PAC-1从第6天开始,H₂组比对照组低（14.89±5.98 vs 19.41±5.17,P<0.05）,差异有统计学意义。Western blotting结果显示从第6天开始,H₂组cleaved Caspase-9和cleaved Caspase-3含量表达比对照组低（P<0.001）,第7天开始,H₂组Bax含量表达比对照组低（P<0.001）,Bcl-2比对照组高（P<0.05）,Bax/Bcl-2的比值比对照组低,差异有统计学意义（P<0.001）。结论 H₂可以抑制单采血小板活化和凋亡,一定程度上改善保存质量。

关键词: 氢气, 血小板保存损伤, 活化, 凋亡

Abstract: Objective To explore the effect and mechanism of hydrogen (H₂) on apheresis platelets activation and apoptosis. Methods 10 apheresis platelets were divided equally into 16 small bags and randomly divided into an H₂ group and a control group. The H₂ group was added with hydrogen to saturation and supplemented with hydrogen daily. All samples were stored under agitation at (22±2) ℃ for 8 days, the platelet count was tested, and the CD62P, PAC-1, apoptosis were detected by flow cytometry daily. The Caspase-3, Caspase-9, Bax, and Bcl-2 proteins were detected by Western blotting. Results There was no significant difference in platelet count during the preservation period (1~8 d). From the fifth day, the apoptosis rate in H₂ group was significantly lower than that in the control group (20.29±4.32 vs 33.06±8.01, P<0.001). From the sixth day, the expression of CD62P in H₂ group was lower than that in the control group (24.94±5.53 vs 47.14±10.41, P<0.001), and the expression of PAC-1 in H₂ group was lower than that in the control group (14.89±5.98 vs 19.41±5.17, P<0.05). Western blotting results showed that from the sixth day, the expression of cleaved Caspase-9 and cleaved Caspase-3 in the H₂ group was lower than that in the control group (P<0.001). From the seventh day, the expression of Bax in the H₂ group was lower than that in the control group (P<0.001), while Bcl-2 was higher than that in the control group (P<0.05), the ratio of Bax/Bcl-2 was lower than that of the control group. Conclusion Hydrogen can reduce platelet activation and apoptosis, improve the quality of apheresis platelet storage.

Key words: Hydrogen, Platelet storage lesion, Activation, Apoptosis

中图分类号:

R331.1⁺43

李津杞, 秦爱华, 任瑜菲, 唐河山, 陈翠敏, 钱宝华, 臧艳. 氢气抑制单采血小板活化和凋亡的作用研究^*[J]. 临床输血与检验, 2025, 27(6): 775-782.

LI Jinqi, QIN Aihua, REN Yufei, TANG Heshan, CHEN Cuimin, QIAN Baohua, ZANG Yan. Inhibitory Effects of Hydrogen on Activation and Apoptosis in Stored Apheresis Platelets[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(6): 775-782.

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氢气抑制单采血小板活化和凋亡的作用研究^*

Inhibitory Effects of Hydrogen on Activation and Apoptosis in Stored Apheresis Platelets

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