苯肼诱导慢性溶血性贫血对外周血及骨髓干细胞影响*

doi:10.3969/j.issn.1671-2587.2026.01.004

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (1): 22-28.DOI: 10.3969/j.issn.1671-2587.2026.01.004

苯肼诱导慢性溶血性贫血对外周血及骨髓干细胞影响^*

李欣蕾, 安宁, 王雅芬, 吴晓双, 陈洁, 何阳, 陈要臻, 胡兴斌

西北大学生命科学学院，空军军医大学第一附属医院输血科，曲江新区医院输血科，陕西西安 710000

收稿日期:2025-05-15 发布日期:2026-02-13
通讯作者: 胡兴斌，主要从事输血医学研究，（E-mail）hxbyqh@163.com。
作者简介:李欣蕾，主要从事生物技术与工程研究，（E-mail）202332787@stumail.nwu.edu.cn。
基金资助:
*本课题受国家重点研究项目（No.2022YFC3400103）资助

Effects of Phenylhydrazine-induced Chronic Hemolytic Anemia on Peripheral Blood and Bone Marrow Stem Cells

LI Xinlei, AN Ning, WANG Yafen, WU Xiaoshuang, CHEN Jie, HE Yang, CHEN Yaozhen, HU Xingbin

Northwest University; Department of Blood Transfusion, Xijing Hospital, Air Force Military Medical University; Department of Blood Transfusion, Qujiang New District Hospital, Xi'an 710000

Received:2025-05-15 Published:2026-02-13

摘要/Abstract

摘要： 目的初步探索苯肼诱导的慢性溶血性贫血病理状态下造血系统多层级调控的影响。方法利用C57BL/6小鼠构建苯肼诱导的慢性溶血性贫血模型，通过检测血常规、游离血红蛋白浓度;流式细胞术检测骨髓中红细胞终末期各阶段细胞、造血干细胞及骨相关间充质干细胞比例变化等指标，分析慢性溶血性贫血对造血系统多层级影响。结果血常规及游离血红蛋白结果显示成功构建苯肼诱导的慢性溶血性贫血模型。流式细胞术结果显示，实验组小鼠早幼红细胞数量显著增加（2.33±0.29）%（P<0.01）及中幼红细胞增加（1.04±0.23）%（P<0.05）;骨髓造血干细胞显著性增加（0.16±0.03）%（P<0.000 1）及骨相关间充质干细胞比例也明显升高。结论苯肼诱导的慢性溶血性贫血可触发造血系统多层级的调控，这为维持机体红细胞平衡，为探索溶血性贫血的发病机制提供理论依据。

关键词: 苯肼, 慢性溶血性贫血, 红细胞, 骨相关间充质干细胞, 骨髓造血系统

Abstract: Objective To investigate the effects of multi-level regulatory effects within the hematopoietic system in a murine model of phenylhydrazine(PHZ)-induced chronic hemolytic anemia. Methods A chronic hemolytic anemia model was established in C57BL/6 mice using PHZ. We analyzed multiple hematopoietic parameters, including complete blood count, plasma-free hemoglobin, and flow cytometric quantification of terminal erythroid differentiation stages, hematopoietic stem cells (HSCs) , and bone marrow mesenchymal stem cells (BMSCs). Results Successful model establishment was confirmed by complete blood count and elevated free hemoglobin. Flow cytometry revealed significant increases in the proportions of proerythroblasts [(2.33±0.29)%, P<0.01] and basophilic erythroblasts [(1.04±0.23)%, P<0.05]. Additionally, the proportions of HSCs [(0.16±0.03)%, P<0.000 1] and BMSCs were significantly elevated in the bone marrow. Conclusion PHZ-induced chronic hemolytic anemia triggers multi-level regulation within the hematopoietic system, which helps maintain erythrocyte homeostasis and provides a theoretical basis for understanding the pathogenesis of hemolytic anemia.

Key words: Phenylhydrazine, Chronic hemolytic anemia, Red blood cell, Bone marrow mesenchymal stem cell, Bone marrow hematopoietic system

中图分类号:

R556.6R329.2

李欣蕾, 安宁, 王雅芬, 吴晓双, 陈洁, 何阳, 陈要臻, 胡兴斌. 苯肼诱导慢性溶血性贫血对外周血及骨髓干细胞影响^*[J]. 临床输血与检验, 2026, 28(1): 22-28.

LI Xinlei, AN Ning, WANG Yafen, WU Xiaoshuang, CHEN Jie, HE Yang, CHEN Yaozhen, HU Xingbin. Effects of Phenylhydrazine-induced Chronic Hemolytic Anemia on Peripheral Blood and Bone Marrow Stem Cells[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(1): 22-28.

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苯肼诱导慢性溶血性贫血对外周血及骨髓干细胞影响^*

Effects of Phenylhydrazine-induced Chronic Hemolytic Anemia on Peripheral Blood and Bone Marrow Stem Cells

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