Th17/Treg平衡及细胞因子水平与血小板输注无效的相关性研究*

doi:10.3969/j.issn.1671-2587.2026.01.010

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (1): 65-69.DOI: 10.3969/j.issn.1671-2587.2026.01.010

Th17/Treg平衡及细胞因子水平与血小板输注无效的相关性研究^*

刘雯¹, 王文星², 范娜¹, 陈伟³, 梁静¹

¹新疆医科大学第六附属医院输血科;
²新疆医科大学第六附属医院;
³新疆维吾尔自治区人民医院输血科，乌鲁木齐 830002

收稿日期:2025-05-12 发布日期:2026-02-13
通讯作者: 梁静，主要从事临床输血学的免疫机制研究，（E-mail）1793645027@qq.com。
作者简介:刘雯，主要从事临床输血学的免疫机制研究，（E-mail）1277879139@qq.com。
基金资助:
*本课题受新疆维吾尔自治区自然科学基金青年基金项目（No.2022D01C822）; 新疆维吾尔自治区自然科学基金面上项目（No.2022D01C585）资助

Imbalance between Th17/Treg Cells and Associated Cytokines in Patients with Platelet Transfusion Refractoriness

LIU Wen¹, WANG Wenxing², FAN Na¹, CHEN Wei³, LIANG Jing¹

¹The Sixth Affiliated Hospital of Xinjiang Medical University, Blood Transfusion Department;
²The Sixth Affiliated Hospital of Xinjiang Medical University;
³People's Hospital of Xinjiang Uygur Autonomous Region, Blood Transfusion Department, Urumqi 830002

Received:2025-05-12 Published:2026-02-13

摘要/Abstract

摘要： 目的探讨免疫性血小板输注无效（IPTR）患者外周血调节性T细胞（Treg）、辅助性T细胞17（Th17）及相关细胞因子的变化特征。方法通过病例对照研究纳入IPTR（n=20）、血小板输注有效患者（n=20）及健康对照（n=20），采用流式细胞术检测Treg（CD4+CD25+Foxp3+）和Th17（CD4+IL-17A+）细胞比例，并通过ELISA测定血清IL-6、IL-10、IL-17A及TGF-β1水平。结果与其他两组相比，IPTR组Treg比例显著降低（P<0.05），Th17比例及Th17/Treg比值显著升高（P<0.05），同时伴随促炎因子IL-6、IL-17A水平升高（P<0.05）及抗炎因子IL-10、TGF-β1水平下降（P<0.05）。结论 IPTR患者存在Treg/Th17轴失衡及促炎-抗炎细胞因子网络失调，提示免疫稳态破坏可能通过驱动炎症反应和抑制免疫耐受参与IPTR病理过程。

关键词: 免疫性血小板输注无效, 调节性T细胞, 辅助性T细胞, 细胞因子, 免疫稳态

Abstract: Objective To investigate the changs and characteristics of regulatory T cells (Treg), helper T cells 17 (Th17) and related cytokines in the peripheral blood of patients with immune platelet transfusion refractoriness (IPTR). Methods A case-control study was conducted involving 20 patients with IPTR, 20 patients with effective platelet transfusion and 20 healthy controls. Flow cytometry was applied to detect the proportions of Treg (CD4+CD25+Foxp3+) and Th17 (CD4+IL-17A+) cells. Enzyme-linked immunosorbent assay (ELISA) was used to measure the serum levels of interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-17A (IL-17A) and transforming growth factor-β1 (TGF-β1). Results Compared with the healthy control group, the IPTR group showed a significantly decreased proportion of Treg (P<0.05), accompanied by a significantly increased proportion of Th17 cells and an elevated Th17/Treg ratio (both P<0.05). Meanwhile, the serum levels of pro-inflammatory cytokines IL-6 and IL-17A were significantly elevated (both P<0.05), while the levels of anti-inflammatory cytokines IL-10 and TGF-β1 were significantly reduced (both P<0.05). Conclusion Patients with IPTR have an imbalance in the Treg/Th17 axis and dysregulation of the pro-inflammatory and anti-inflammatory cytokine network. This finding suggests that the disruption of immune homeostasis may contribute to the pathogenesis of PTR by enhancing inflammatory responses and impairing immune tolerance.

Key words: Immune platelet transfusion refractoriness, Regulatory T cells, Helper T cells, Cytokines, Immune homeostasis

中图分类号:

R457.1

刘雯, 王文星, 范娜, 陈伟, 梁静. Th17/Treg平衡及细胞因子水平与血小板输注无效的相关性研究^*[J]. 临床输血与检验, 2026, 28(1): 65-69.

LIU Wen, WANG Wenxing, FAN Na, CHEN Wei, LIANG Jing. Imbalance between Th17/Treg Cells and Associated Cytokines in Patients with Platelet Transfusion Refractoriness[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(1): 65-69.

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Th17/Treg平衡及细胞因子水平与血小板输注无效的相关性研究^*

Imbalance between Th17/Treg Cells and Associated Cytokines in Patients with Platelet Transfusion Refractoriness

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