4 ℃冷藏保存血小板输注对大鼠失血性休克/复苏致急性肾损伤的影响研究

doi:10.3969/j.issn.1671-2587.2024.04.007

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (4): 470-474.DOI: 10.3969/j.issn.1671-2587.2024.04.007

4 ℃冷藏保存血小板输注对大鼠失血性休克/复苏致急性肾损伤的影响研究

谯铭铭, 贡觉顿珠, 陈元锋, 于媛, 叶辉, 庄云龙

山东省血液中心质量管理科,山东济南 250014

收稿日期:2024-07-09 出版日期:2024-08-20 发布日期:2024-09-23
通讯作者: 庄云龙,主要从事血小板储存相关分子机制研究,（E-mail）happymike110@126.com。
作者简介:谯铭铭,主要从事血小板储存相关机制研究与动物模型研究,（E-mail）qiaohcg@163.com。

Effect of 4 ℃ Cold-Stored Platelets Transfusion on Acute Renal Injury Induced by Hemorrhagic Shock in Rats

QIAO Mingming, Gongjuedunzhu, CHEN Yuanfeng, YU Yuan, YE Hui, ZHUANG Yunlong

Blood center of Shandong province, Ji’nan 250014

Received:2024-07-09 Online:2024-08-20 Published:2024-09-23

摘要/Abstract

摘要： 目的研究4 ℃冷藏保存血小板（cold storage platelets,CSP）对大鼠失血性休克/复苏( HS/R)导致急性肾损伤的影响。方法健康雄性SD大鼠40只,随机选取20只用以制备血小板制剂,剩余大鼠随机分为4组：假手术组（空白组）、HS/R模型组（模型组）、CSP输注组、22 ℃±2 ℃振荡保存血小板输注组（RTP输注组）。容量复苏后24 h处死动物,采集血和肾组织标本。检测各组血清中血肌酐（Scr）、尿素氮(BUN)、血清胱抑素C（CysC）及肾组织中白细胞介素1β（IL-1β）、白细胞介素18（IL-18）含量。Western Blot法检测各组大鼠肾组织中性粒细胞明胶酶相关脂质运载蛋白（NGAL）、肾损伤因分子1（KIM-1）表达量,HE染色观察肾组织病理学改变。结果 ①四组大鼠基线状态体质量、MAP、SCr、BUN 等指标无明显差异（P>0.05）。②复苏后24 h,模型组大鼠SCr（P<0.05）、BUN（P<0.01）、CysC（P<0.01）水平均高于其他三组;CSP输注组BUN（P<0.01）、SCr（P=0.037）、CysC（P=0.020）水平低于RTP输注组。③与模型组比较,CSP输注组和RTP输注组血清IL-1β和IL-18 水平均显著降低。较于RTP输注组,CSP输注组血清IL-1β水平有所降低(P=0.041)。④与模型组相比,CSP输注组NGAL 蛋白表达明显下降（P<0.01）,CSP输注组和RTP输注组KIM-1蛋白表达均显著下降（P<0.01）。肾组织病理学结果示,CSP输注可减轻肾细胞变性、坏死及炎性细胞浸润程度。结论较于RTP,CSP输注可减轻SD大鼠HS/R急性肾损伤。

关键词: 4 ℃冷藏保存血小板, 失血性休克, 急性肾损伤

Abstract: Objectives To investigate the effects of 4 ℃ cold-stored platelets (cold storage platelets, CSP) transfusion on acute kidney injury caused by hemorrhagic shock/resuscitation (HS/R) in rats. Methods 20 healthy male SD rats were randomly divided into 4 groups: sham operation group (blank group), HS/R model group (model group), CSP transfusion group, room temperature-stored platelets (RTP) kept at 22 ℃ transfusion group (RTP transfusion group). The rats were executed 24 hours after volume resuscitation, and blood and kidney tissue specimens were collected. Serum concentrations of creatinine (Scr), urea nitrogen (BUN) , cystatin C (CysC) and interleukin-1β (IL-1β) and interleukin-18 (IL-18) in renal tissue were detected in each group. The expression levels of neutrophil gelatinase-associated lipid carrier protein (NGAL) and renal injury cause-1 (KIM-1) in renal tissue were detected by Western Blot method, and the pathological changes of renal tissue were observed by HE staining. Results ①There were no significant differences in baseline body mass, MAP, SCr, BUN and other indexs among four groups (P>0.05). ②24 h after resuscitation, the levels of SCr (P<0.05), BUN (P<0.01), CysC (P<0.01) were higher in the model group than in the other three groups; the levels of BUN (P<0.01), SCr (P=0.037), CysC (P=0.020) in the CSP infusion group were lower than those in the RTP infusion group. ③Compared with the model group, serum IL-1β and IL-18 levels were significantly lower in both CSP infusion group and RTP infusion group. Compared with the RTP infusion group, serum IL-1β levels were reduced in the CSP infusion group (P=0.041). Compared with model group, NGAL protein expression was significantly decreased in CSP infusion group (P<0.01), and KIM-1 protein expression was significantly decreased in both CSP infusion group and RTP infusion group (P<0.01). Renal histopathological results showed that CSP transfusion could reduce the degree of renal cell degeneration, necrosis and inflammatory cell infiltration. Conclusion Compared with RTP, CSP transfusion could reduce HS/R acute kidney injury in SD rats.

Key words: 4 ℃ cold-stored platelets, Haemorrhagic shock, Acute kidney injury

中图分类号:

R457

谯铭铭, 贡觉顿珠, 陈元锋, 于媛, 叶辉, 庄云龙. 4 ℃冷藏保存血小板输注对大鼠失血性休克/复苏致急性肾损伤的影响研究[J]. 临床输血与检验, 2024, 26(4): 470-474.

QIAO Mingming, Gongjuedunzhu, CHEN Yuanfeng, YU Yuan, YE Hui, ZHUANG Yunlong. Effect of 4 ℃ Cold-Stored Platelets Transfusion on Acute Renal Injury Induced by Hemorrhagic Shock in Rats[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(4): 470-474.

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4 ℃冷藏保存血小板输注对大鼠失血性休克/复苏致急性肾损伤的影响研究

Effect of 4 ℃ Cold-Stored Platelets Transfusion on Acute Renal Injury Induced by Hemorrhagic Shock in Rats

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