AMPK信号通路在小鼠急性肾损伤发生中的作用

doi:10.3969/j.issn.1671-2587.2021.04.008

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (4): 446-449.DOI: 10.3969/j.issn.1671-2587.2021.04.008

AMPK信号通路在小鼠急性肾损伤发生中的作用

毛艳, 卢丽, 肖辉, 刘潇

442008 湖北医药学院附属国药东风总医院肾内科

收稿日期:2019-12-18 发布日期:2021-08-18
通讯作者: 卢丽,女,主要从事肾脏内科和血液净化专科护理,（E-mail）luliluli36@163.com。
作者简介:毛艳（1984-）,女,湖北十堰人,主治医师,硕士,主要从事肾脏病研究,（E-mail）maoyan3225@163.com。

Role and Mechanism of AMPK Signaling Pathway in the Development of Acute Kidney Injury in Mice

MAO Yan, LU Li, XIAO Hui, et al

Department of Nephrology, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008,Hubei,china

Received:2019-12-18 Published:2021-08-18

摘要/Abstract

摘要： 目的探讨磷酸腺苷激活蛋白酶（adenosine phosphate activating protease,AMPK）信号通路在小鼠急性肾损伤发生中的作用。方法 SPF级BALB/c雄性小鼠（48只）随机分为两组,对照组与实验组各24只。实验组采用阿德福韦酯40 mg/kg灌胃处理,1次/周,持续2次;对照组用等量等渗盐水进行灌胃处理。采用全自动生化分析仪测定血清尿素氮（BUN）与肌酐（SCr）含量,采用Western blotting检测肾组织中AMPK、Keap-1的表达,同时进行组织病理学评分。结果处理2周、4周、6周后,实验组的血清BUN与SCr水平都显著高于对照组（P<0.05）;实验组的肾脏组织病理学评分都显著高于对照组（P<0.05）;实验组的肾脏AMPK与Keap-1蛋白相对表达水平都显著高于对照组（P<0.05）。结论小鼠急性肾损伤可激活AMPK信号通路,诱导AMPK与Keap-1表达量上升,导致肾脏组织损伤与肾功能下降。

关键词: 小鼠, 急性肾损伤, 磷酸腺苷激活蛋白酶, 肾功能

Abstract: Objective To investigate the role and mechanism of Adenosine phosphate activating protease （AMPK） signaling pathway in the development of acute kidney injury in mice. Method 48 SPF BALB/C male mice were randomly divided into two groups：control group（n=24） and experimental group（n=24）. The experimental group were treated with adefovir dipivoxil 40 mg/kg,once a week for 2 times,while the control group were treated with equal amount of isotonic saline. Serum urea nitrogen（BUN）and creatinine（SCr） were measured by automatic biochemical analyzer. The expression of AMPK and Keap-1 in renal tissue were detected by Western blotting,and histopathological scores were also obtained. Results After 2 weeks,4 weeks,and 6 weeks of treatment,serum BUN and SCr values in the experimental group were significantly higher than those in the control group（P<0.05）,the histopathological scores of the kidneys in the experimental group were significantly higher than those in the control group （P<0.05）,and the relative expression levels of AMPK and Keap-1 protein in the kidneys of the experimental group were significantly higher than those in the control group （P<0.05）. Conclusion Acute kidney injury in mice can activate the AMPK signaling pathway and induce the expression of AMPK and Keap-1 increase,which leads to the deterioration of renal tissue and renal function.

Key words: Mice, Acute kidney injury, Adenosine phosphate activating protease, Renal function

中图分类号:

R692

毛艳, 卢丽, 肖辉, 刘潇. AMPK信号通路在小鼠急性肾损伤发生中的作用[J]. 临床输血与检验, 2021, 23(4): 446-449.

MAO Yan, LU Li, XIAO Hui, et al. Role and Mechanism of AMPK Signaling Pathway in the Development of Acute Kidney Injury in Mice[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(4): 446-449.

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AMPK信号通路在小鼠急性肾损伤发生中的作用

Role and Mechanism of AMPK Signaling Pathway in the Development of Acute Kidney Injury in Mice

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