利用细胞化学染色方法和原位杂交技术探讨红细胞中核酸物质表达及定位情况*

doi:10.3969/j.issn.1671-2587.2025.03.008

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (3): 325-328.DOI: 10.3969/j.issn.1671-2587.2025.03.008

利用细胞化学染色方法和原位杂交技术探讨红细胞中核酸物质表达及定位情况^*

孙莉萍, 曹腾宇, 段翠密, 于洋

中国人民解放军总医院第一医学中心输血医学科, 北京 100853

收稿日期:2024-12-16 发布日期:2025-06-23
通讯作者: 于洋,主要从事单采治疗、野战输血及智能化输血辅助决策系统研究,（E-mail）yuyangpla301@163.com。
作者简介:孙莉萍,主要从事输血医学基础研究,（E-mail）sunlp2012@sina.com。
基金资助:
*本课题受国家自然科学基金面上项目（No.81970167）资助

Exploring the Expression and Localization of Nucleic Acid Substances in Red Blood Cells Using Cellular Chemical Staining Methods and in Situ Hybridization Techniques

SUN Liping, CAO Tengyu, DUAN Cuimi, YU Yang

Department of Transfusion Medicine, The First Medical Center, Chinese PLA General Hospital, Beijing 100853

Received:2024-12-16 Published:2025-06-23

摘要/Abstract

摘要： 目的探索红细胞中核酸物质的表达及定位情况。方法首先,选取健康人红细胞作为实验样本（A549有核细胞系为对照）,利用细胞化学染色方法和原位杂交技术,对其中DNA和RNA进行相关检测,明确红细胞中核酸物质的表达情况。其次,提取红细胞微囊泡（microvesicle, MV）,并将其与吞噬能力强的小鼠单核巨噬细胞系RAW264.7共培养,利用原位杂交染色方法检测受体细胞RAW264.7中红细胞特异性miRNA（miR-451a）的定位情况。结果红细胞中存在核酸物质（DNA和RNA）,并且红细胞中存在高表达量的miR-451a,提示红细胞中的miR-451a可能通过MV进入受体细胞。结论利用细胞化学染色方法和原位杂交技术可以检测到红细胞中存在核酸物质。

关键词: 红细胞, 核酸物质, miR-451a, 细胞化学染色, 原位杂交技术

Abstract: Objective To explore the expression and localization of nucleic acid substances in red blood cells (RBCs). Methods Firstly, healthy human RBCs were selected as experimental samples (A549 nucleated cell line as control). Cellular chemical staining methods and in situ hybridization techniques were used to detect DNA and RNA, and to clarify the expression of nucleic acid substances in RBCs. Secondly, We extracted microvesicles (MVs) derived from RBCs and co-cultured them with the highly phagocytic mouse monocyte macrophage line RAW264.7 to detect the localization of RBC specific miRNA (miR-451a) in the receptor cell RAW264.7 by in situ hybridization staining. Results Our test results show that there are nucleic acid substances (DNA and RNA) in RBCs. And there exists miR-451a with high expression levels in RBCs, miR-451a in RBCs may enter the receptor cells via MVs. Conclusion The presence of nucleic acid substances in RBCs can be detected using cellular chemical staining methods and in situ hybridization techniques.

Key words: Red blood cells, Nucleic acid, miR-451a, Cellular chemical staining methods, In situ hybridization techniques

中图分类号:

Q2-33R331.1⁺41

孙莉萍, 曹腾宇, 段翠密, 于洋. 利用细胞化学染色方法和原位杂交技术探讨红细胞中核酸物质表达及定位情况^*[J]. 临床输血与检验, 2025, 27(3): 325-328.

SUN Liping, CAO Tengyu, DUAN Cuimi, YU Yang. Exploring the Expression and Localization of Nucleic Acid Substances in Red Blood Cells Using Cellular Chemical Staining Methods and in Situ Hybridization Techniques[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(3): 325-328.

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利用细胞化学染色方法和原位杂交技术探讨红细胞中核酸物质表达及定位情况^*

Exploring the Expression and Localization of Nucleic Acid Substances in Red Blood Cells Using Cellular Chemical Staining Methods and in Situ Hybridization Techniques

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