致敏红细胞BPGM酶活性降低对红细胞携氧/释氧能力的影响研究*

doi:10.3969/j.issn.1671-2587.2024.03.003

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (3): 308-314.DOI: 10.3969/j.issn.1671-2587.2024.03.003

致敏红细胞BPGM酶活性降低对红细胞携氧/释氧能力的影响研究^*

孟炜程, 陈要臻, 王雅芬, 安宁, 刘志新, 吴晓双, 王斐, 胡兴斌

空军军医大学第一附属医院输血科,陕西西安 710032

收稿日期:2024-02-26 出版日期:2024-06-20 发布日期:2024-06-24
通讯作者: 胡兴斌,主要从事输血医学研究,（E-mail）hxbyqh@163.com。
作者简介:孟炜程,主要从事临床检验诊断学,（E-mail）mf1151483515@163.com。
基金资助:
*国家自然科学基金（No.82370230）,陕西省自然科学基础研究计划（No.2021JM-217）资助

The Impact of Reduced Bisphosphoglycerate Mutase（BPGM）Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity

MENG Weicheng, CHEN Yaozhen, WANG Yafen, AN Ning, LIU Zhixin, WU Xiaoshuang, WANG Fei, HU Xingbin

Department of Blood Transfusion, Xijing Hospital, Air Force Military Medical University, Xi'an 710032

Received:2024-02-26 Online:2024-06-20 Published:2024-06-24

摘要/Abstract

摘要： 目的分析致敏红细胞的携氧/释氧能力并初步探讨出现异常的原因。方法用微柱凝胶卡检测致敏红细胞,用携氧/释氧功能评价装置分析自身免疫性溶血性贫血（AIHA）和新生儿溶血病（HDN）患者的致敏红细胞的携氧/释氧能力。使用健康成人的血浆构建输血性溶血反应小鼠模型,对照组给予PBS,记录小鼠负重游泳至力竭的时间,分离小鼠红细胞并分析其携氧/释氧能力,利用蛋白组学对小鼠溶血组与正常组血浆中差异蛋白进行GO和KEGG富集分析。采用ELISA方法检测致敏红细胞BPGM酶活性和2,3-DPG含量。结果与相应对照组相比,AIHA、HDN和输血性溶血反应模型小鼠的红细胞的氧解离曲线均发生左移和P50下降。溶血模型小鼠的负重游泳时间明显缩短,蛋白组学筛选结果发现红细胞BPGM异常,致敏红细胞BPGM酶活性降低且细胞内2,3-DPG含量下降。结论致敏红细胞BPGM酶活性降低导致红细胞释放氧气能力下降。

关键词: 红细胞, 溶血, BPGM, 2,3-DPG

Abstract: Objective To explore the underlying causes for the observed anomalies, the oxygen-carrying/releasing capacity of sensitized red blood cells (RBCs) was analyzed . Methods The sensitized red blood cells were detected through microcolumn gel cards, and an oxygen-carrying/releasing assessment device was employed to evaluate the oxygen-carrying/releasing capacity of sensitized RBCs in patients with autoimmune hemolytic anemia (AIHA) and hemolytic disease of the newborn (HDN). We constructed a transfusion-induced hemolytic reaction mouse model using plasma from healthy adults, while the control group received PBS. Record the time until exhaustion during weight-loaded swimming in mice and analyze the oxygen-carrying/releasing capacity of isolated mouse RBCs. Perform GO and KEGG enrichment analysis on differentially expressed proteins related to hemolysis and the control group using proteomics. Measure BPGM enzyme activity and 2,3-DPG levels in sensitized RBCs using ELISA. Results Compared to the corresponding control groups, the oxygen dissociation curves in AIHA, HDN, and transfusion-induced hemolytic reaction model mice exhibited a left shift and decreased P50. The weight-loaded swimming time in hemolytic model mice was significantly shortened. Proteomic screening revealed abnormalities in BPGM, with decreased BPGM enzyme activity and a decline in intracellular 2,3-DPG content. Conclusion The reduced activity of BPGM in sensitized RBCs led to a decline in their oxygen-releasing capacity.

Key words: Red blood cells, Hemolysis, BPGM, 2,3-DPG

中图分类号:

R457

孟炜程, 陈要臻, 王雅芬, 安宁, 刘志新, 吴晓双, 王斐, 胡兴斌. 致敏红细胞BPGM酶活性降低对红细胞携氧/释氧能力的影响研究^*[J]. 临床输血与检验, 2024, 26(3): 308-314.

MENG Weicheng, CHEN Yaozhen, WANG Yafen, AN Ning, LIU Zhixin, WU Xiaoshuang, WANG Fei, HU Xingbin. The Impact of Reduced Bisphosphoglycerate Mutase（BPGM）Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(3): 308-314.

参考文献

[1] REMIGANTE A,MORABITO R,MARINO A.Band 3 protein function and oxidative stress in erythrocytes[J].J Cell Physiol,2021,236(9):6225-6234.
[2] GIARDINA B.Hemoglobin:multiple molecular interactions and multiple functions.An example of energy optimization and global molecular organization[J].Mol Aspects Med,2022,84:101040.
[3] Disorders of hemoglobin:genetics,pathophysiology,and clinical management[J].J R Soc Med,2001,94(11):602-603.
[4] BULOW L,ALAYASH A I.Redox chemistry of hemoglobin-associated disorders[J].Antioxid Redox Signal,2017,26(14):745-747.
[5] CAPRIOTTI T,FRIZZELL J P.Davis Advantage for Pathophysiology: Introductory Concepts and Clinical Perspectives[M].Philadelphia: F.A.Davis Company,2016.
[6] SIDDON A J,TORMEY C A.The chemical and laboratory investigation of hemolysis[J].Adv Clin Chem,2019,89:215-258.
[7] JAMWAL M,SHARMA P,DAS R.Laboratory approach to hemolytic Anemia[J].Indian J Pediatr,2020,87(1):66-74.
[8] FAR M A J,EFTEKHAR Z,FAR M A J,et al.Red Blood Cell Alloimmunization: Life-Threatening Response[M/OL].(2024-03-21).https://www.intechopen.com/online-first/1163707.
[9] SAWITSKY A,JR OZAETA P B.Disease-associated autoimmune hemolytic anemia[J].Bull N Y Acad Med,1970,46(6):411-426.
[10] D'ALESSANDRO A,XIA Y.Erythrocyte adaptive metabolic reprogramming under physiological and pathological hypoxia[J].Curr Opin Hematol,2020,27(3):155-162.
[11] SUN K Q,ZHANG Y J,D'ALESSANDRO A,et al.Sphingosine-1-phosphate promotes erythrocyte glycolysis and oxygen release for adaptation to high-altitude hypoxia[J].Nat Commun,2016,7:12086.
[12] XU P,CHEN C H,ZHANG Y J,et al.Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis[J].Cell Metab,2022,34(2):299-316.e6.
[13] LIU H,ZHANG Y J,WU H Y,et al.Beneficial role of erythrocyte adenosine A2B receptor-mediated AMP-activated protein kinase activation in high-altitude hypoxia[J].Circulation,2016,134(5):405-421.
[14] CHEN C H,XIE T T,ZHANG Y J,et al.Erythrocyte ENT1-AMPD3 axis is an essential purinergic hypoxia sensor and energy regulator combating CKD in a mouse model[J].J Am Soc Nephrol,2023,34(10):1647-1671.
[15] 刘志新,吴晓双,孟炜程,等.一种急性溶血性输血反应小鼠动物模型的建立[J].临床输血与检验,2023,25(1):63-69.
[16] 黄红,姜宁,吕静薇,等.不同时间睡眠干扰致小鼠体力疲劳模型的建立[J].中国比较医学杂志,2019,29(1):16-20.
[17] MADIGAN M T.Brock biology of microorganisms[M].13th ed.Boston:Benjamin Cummings,2012.
[18] MCMULLIN M F.Congenital erythrocytosis[J].Int J Lab Hematol,2016,38(Suppl 1):59-65.
[19] VLACHADIS N,VRACHNIS N.A review of sickle cell disease[J].JAMA,2022,328(19):1979.
[20] PERROTTA S,GALLAGHER P G,MOHANDAS N.Hereditary spherocytosis[J].Lancet,2008,372(9647):1411-1426.
[21] 陈秀丽,刘军贤,申卫东,等.喇曼光镊分析血红细胞的携氧能力[J].光子学报,2010,39(5):907-912.
[22] CHEN Y,CHEN S,LIU Z,et al.Noncanonical NLRP3 Inflammasome Activation Elicits the Programmed Death of Red Blood Cells[J/OL].(2023-11-08).https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4626203.

致敏红细胞BPGM酶活性降低对红细胞携氧/释氧能力的影响研究^*

The Impact of Reduced Bisphosphoglycerate Mutase（BPGM）Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

在线期刊

访问统计

联系我们

[1]	红细胞血型抗原拓展匹配适用范围中国专家共识编写组. 红细胞血型抗原拓展匹配适用范围中国专家共识[J]. 临床输血与检验, 2024, 26(3): 289-298.
[2]	陈婷婷, 黄蓉, 凌玉, 胡文静. RhD阴性孕产妇与HDFN发生的相关性及影响因素分析^*[J]. 临床输血与检验, 2024, 26(3): 338-345.
[3]	甘丽, 易平, 赵勤俭, 张馨元. 基于红细胞的疫苗载体研究进展^*[J]. 临床输血与检验, 2024, 26(3): 404-411.
[4]	吴常睿, 黄远帅, 王洁. 吸烟对红细胞影响的相关研究进展^*[J]. 临床输血与检验, 2024, 26(3): 411-419.
[5]	中国输血协会临床输血管理学专业委员会. 术前深度单采自体红细胞和/或血小板储血技术应用中国专家共识[J]. 临床输血与检验, 2024, 26(2): 145-155.
[6]	范瑞, 封春雨, 王雨婧, 尚玮玮, 唐文飞, 马海梅, 张欢. 体外模拟加压输血对悬浮红细胞质量影响的研究^*[J]. 临床输血与检验, 2024, 26(2): 223-229.
[7]	葛晓芹, 付恒, 胡官林, 曹彩霞, 杨维涛, 王振兴, 周国琼, 付竹筠, 孙婧雯, 沈有华, 燕锋, 鲍琳, 林富文, 李丽, 王泽颖, 冯兰洁, 周竞, 康美艳, 张剑, 刘保霞, 汤文隽, 郭萍莉, 李浩, 邱艳. 新冠肺炎疫情对国内25家地级市中心血站全血制备成分血的供应影响分析^*[J]. 临床输血与检验, 2024, 26(2): 244-250.
[8]	李洋, 杨明峰, 王莹, 张坚, 刘嘉馨, 孙保亮. 血红蛋白类氧载体的研究进展及应用前景^*[J]. 临床输血与检验, 2024, 26(2): 282-288.
[9]	中国输血协会临床输血管理学专业委员会. 婴儿期输血前红细胞血型血清学检测中国专家共识^*[J]. 临床输血与检验, 2024, 26(1): 8-16.
[10]	阳雪新, 袁咏梅, 吴兆勇, 苑召虎, 刘珍, 童欣欣, 陈小卫, 魏亚明. ABO次侧不相合血小板输注的有效性和安全性初步研究^*[J]. 临床输血与检验, 2024, 26(1): 42-48.
[11]	尹明秀, 欧国进, 陈剑, 凤婧, 赵虹. 改良56℃热放散法与冷冻复融放散法对ABO型新生儿溶血病的临床应用价值比较^*[J]. 临床输血与检验, 2024, 26(1): 60-65.
[12]	杨晓樨, 赵营营, 马吉芳, 于政. 硅芯片纳米微流体技术与磁激活细胞分选法对胎儿有核红细胞富集效果的比较[J]. 临床输血与检验, 2024, 26(1): 86-91.
[13]	尤来委, 姚娜, 吴曼迪, 张岩, 崔应辉, 郭建荣. 不同配方保存液对2型糖尿病患者贮存式自体红细胞保护作用的对比研究^*[J]. 临床输血与检验, 2023, 25(6): 721-726.
[14]	陈旭升, 杨永涛. 静脉输注丙种球蛋白对非新生儿溶血患儿交叉配血的影响[J]. 临床输血与检验, 2023, 25(6): 749-752.
[15]	陈源, 张晓丹, 叶若婷, 吴伟祥, 刘成义. ABO-HDN患儿血型IgG抗体效价及亚型的分布特征分析^*[J]. 临床输血与检验, 2023, 25(6): 753-757.