硅芯片纳米微流体技术与磁激活细胞分选法对胎儿有核红细胞富集效果的比较

doi:10.3969/j.issn.1671-2587.2024.01.014

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (1): 86-91.DOI: 10.3969/j.issn.1671-2587.2024.01.014

硅芯片纳米微流体技术与磁激活细胞分选法对胎儿有核红细胞富集效果的比较

杨晓樨¹, 赵营营¹, 马吉芳¹, 于政²

¹辽阳市中心医院检验科,辽宁辽阳 111000;
²陆军第79集团军医院检验科,辽宁盘锦 111000

收稿日期:2023-11-09 发布日期:2024-03-13
作者简介:杨晓樨,主要从事血液细胞形态异常血液细胞形态病历分析,（E-mail）yanghpqa@163.com。

Comparison of Enrichment Effects of Silicon Chip Nanomicrofluidic Technology and Magnetic Activated Cell Sorting Method on Fetal Nucleated Red Blood Cells

YANG Xiaoxi¹, ZHAO Yingying¹, MA Jifang¹, YU Zheng²

¹Laboratory Department of Liaoyang Central Hospital LiaoNing Liaoyang 111000;
²Laboratory Department of The 79th Army Group Hospital of the Army, LiaoNing Panjin 111000

Received:2023-11-09 Published:2024-03-13

摘要/Abstract

摘要： 目的分析硅芯片纳米微流体技术与磁激活细胞分选法对胎儿有核红细胞（FNRBC）的富集效果。方法选取2020年1月—2022年12月拟于医院产科门诊进行产前筛查的112例孕妇为研究对象,所有孕妇均采集外周肘静脉血10 mL,分别通过硅芯片纳米微流体技术与磁激活细胞分选法对血液样本中FNRBC进行富集。比较两种方案富集前后细胞形态及细胞计数情况,记录两组方案对FNRBC的富集时间及富集所得FNRBC的无菌试验结果。结果硅芯片纳米微流体技术对FNRBC的富集时间长于磁激活细胞分选法（P<0.05）;两种方案的无菌试验阳性发生率比较,差异无统计学意义（P>0.05）;与磁激活细胞分选法相比,硅芯片纳米微流体技术进行FNRBC富集时所得的总细胞量较少,FNRBC量较多,FNRBC比例较高（P<0.05）。结论硅芯片纳米微流体技术与磁激活细胞分选法进行FNRBC富集所得样本受污染风险均较低,与磁激活细胞分选法相比,硅芯片纳米微流体技术去除混杂细胞的能力较强,且获得的FNRBC数量较多,对FNRBC的富集效果较好,但所需时间较长。

关键词: 胎儿, 有核红细胞, 硅芯片纳米微流体技术, 磁激活细胞分选法

Abstract: Objective To analyze the effect of silicon chip nanomicrofluidic technology and magnetic activated cell sorting method on the enrichment of fetal nucleated red blood cells (FNRBCs). Method A total of 112 pregnant women who were scheduled to undergo prenatal screening at the hospital's obstetric clinic from January 2020 to December 2022 were selected as the study subjects. 10 mL of blood was collected from the elbow vein and FNRBCs in blood samples were enriched by silicon chip nanomicrofluidic technology and magnetic activated cell sorting method, respectively. We compared the cell morphology and cell count before and after enrichment, the enrichment time of FNRBCs and the sterility test results of two methods. Results The enrichment time of FNRBC by silicon chip nanomicrofluidic technology was longer than that by magnetic activated cell sorting method (P<0.05). There was no statistically significant difference in the positive incidence of sterility tests between the two methods (P>0.05). Compared with the magnetic activated cell sorting method, the silicon chip nanomicrofluidic technology yielded fewer total cells, more FNRBCs, and a higher proportion of FNRBCs (P<0.05). Conclusion The risk of contamination of the samples obtained from FNRBCs enrichment by both methods. Compared with magnetic activated cell sorting method, silicon chip nanomicrofluidic technology was more capable of removing mixed cells and obtains a larger number of FNRBCs, which was more effective in the enrichment of FNRBCs, but it took a longer time.

Key words: Fetus, Nucleated red blood cells, Silicon chip nanomicrofluidic technology, Magnetic activated cell sorting method

中图分类号:

R715.2

杨晓樨, 赵营营, 马吉芳, 于政. 硅芯片纳米微流体技术与磁激活细胞分选法对胎儿有核红细胞富集效果的比较[J]. 临床输血与检验, 2024, 26(1): 86-91.

YANG Xiaoxi, ZHAO Yingying, MA Jifang, YU Zheng. Comparison of Enrichment Effects of Silicon Chip Nanomicrofluidic Technology and Magnetic Activated Cell Sorting Method on Fetal Nucleated Red Blood Cells[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(1): 86-91.

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硅芯片纳米微流体技术与磁激活细胞分选法对胎儿有核红细胞富集效果的比较

Comparison of Enrichment Effects of Silicon Chip Nanomicrofluidic Technology and Magnetic Activated Cell Sorting Method on Fetal Nucleated Red Blood Cells

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