运用抗体效价检测技术鉴定自免溶贫患者的抗体特异性

doi:10.3969/j.issn.1671-2587.2022.01.014

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (1): 63-66.DOI: 10.3969/j.issn.1671-2587.2022.01.014

运用抗体效价检测技术鉴定自免溶贫患者的抗体特异性

高明, 金沙, 向东, 杨佳璇, 雷棽, 宋晨梦, 沈伟

201499 上海市奉贤区,上海市奉贤区中心医院输血科（高明）; 上海血液中心（金沙,向东,杨佳璇,沈伟）; 上海复旦大学附属中山医院厦门医院（雷棽）; 上海长征医院（宋晨梦）

收稿日期:2021-07-24 发布日期:2022-01-27
通讯作者: 沈伟,男,副主任技师,主要从事免疫血液学相关研究,（E-mail）daniels@sh163.net。
作者简介:高明（1979-）,男,上海人,主管技师,本科,主要从事临床输血及血液免疫相关研究,（E-mail）2538702092@qq.com。

Antibody Titration for Antibody Specificity in Patients with Autoimmune Hemolytic Anemia

Gao Ming, Jin Sha, Xiang Dong, et al

Department of Blood Transfusion,South Hospital of the Sixth Affiliated People's Hospital of Shanghai Jiao Tong University,Shanghai 201499

Received:2021-07-24 Published:2022-01-27

摘要/Abstract

摘要： 目的应用抗体效价检测技术检测自免溶贫患者血样中的同种抗体和类同种抗体。方法收集上海市血液中心血型参比实验室2021年3月~4月临床上直抗阳性患者血液样本,使用血清、放散液原液和抗体效价标定的放散液稀释液进行抗体鉴定。结果血清检出4例有特异性抗体,放散液原液检出3例有特异性抗体,放散液稀释液检出9例有特异性抗体。结论直抗阳性患者的放散液稀释液检出特异性的抗体比例高于患者的血清和放散液原液,而抗体效价的检测对于稀释放散液的具体倍数起到决定的作用。抗体效价检测可提高自免溶贫患者类抗体的检出率,可减少由于类抗体特异性导致的输血效果降低,提高输血安全性和有效性。

关键词: 放散液, 效价, 同种抗体, 类同种抗体

Abstract: Objective To identify specific antibodies（allo-antibody and mimicking antibody）in patients with autoimmune hemolytic anemia（AIHA）by antibody titration procedure. Methods Samples from patients tested positive for direct antiglobulin test from the blood group reference laboratory of Shanghai Blood Center between March and April 2021 were collected. Serum,eluate and diluent eluate for antibody titers were used to identify antibodies. Results Four specific antibodies in serum,3 specific antibodies in eluate,9 specific antibodies in diluent eluate were detected. Conclusion The proportion of specific antibodies detected in diluent eluate in positive DAT patients were higher than that of serum and eluate. The antibody titers play a decisive role in eluate dilution. Testing for antibody titer can increase detection rate of mimicking antibody in AIHA patients. It can reduce the reduction of blood transfusion effect due to mimicking antibody to improve the safety and effectiveness of a transfusion.

Key words: Eluate Antibody titer, Allo-antibody, Mimicking antibody

中图分类号:

R457

高明, 金沙, 向东, 杨佳璇, 雷棽, 宋晨梦, 沈伟. 运用抗体效价检测技术鉴定自免溶贫患者的抗体特异性[J]. 临床输血与检验, 2022, 24(1): 63-66.

Gao Ming, Jin Sha, Xiang Dong, et al. Antibody Titration for Antibody Specificity in Patients with Autoimmune Hemolytic Anemia[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(1): 63-66.

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运用抗体效价检测技术鉴定自免溶贫患者的抗体特异性

Antibody Titration for Antibody Specificity in Patients with Autoimmune Hemolytic Anemia

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