• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (1): 71-74.DOI: 10.3969/j.issn.1671-2587.2016.01.021

• 质量控制研究 • 上一篇    下一篇

汇集浓缩血小板滤除白细胞前后质量检测分析

高加良,雷宇,万浬科,王红,袁理   

  1. 610041 四川省成都市血液中心(高加良,雷宇,万浬科); 中国医学科学院,北京协和医学院,输血研究所(王红,袁理)
  • 收稿日期:2015-08-22 出版日期:2016-02-20 发布日期:2016-10-10
  • 作者简介:高加良(1970-),男,四川成都人,副主任技师,硕士,主要从事血液检验及质量管理,(Tel)028-85588705(E-mail)gaojialiang18@sina.com。

The Quality Indicator Analysis of Pooled Platelet Concentrates after Removal of Leukocytes

GAO Jialiang, LEI Yu, WAN Li-ke, et al   

  1. Chengdu Blood Center, Chengdu 610041
  • Received:2015-08-22 Online:2016-02-20 Published:2016-10-10

摘要: 目的开展全血制备浓缩血小板的汇集及滤除白细胞的研究,为临床提供该血液品种提供质量保证的依据。方法采用富含血小板血浆法由400 ml新鲜全血制备浓缩血小板,并将6袋ABO同型的浓缩血小板汇集成1 U成人治疗量,用国产血小板型去白细胞滤器进行过滤,检测过滤前后血小板的相关质量指标。结果汇集血小板过滤前后的血小板数、红细胞混入量、白细胞混入量、pH值、低渗休克反应值分别为:(3.20±0.37)×1011、(2.67±0.44)×1011、(4.39±1.97)×109、(3.95±1.77)×109、(2.06±2.02)108、(0.02±0.03)×108、6.78±1.18、6.98±1.06、(68.53±9.18)%、(70.96±7.81)%; 过滤后血小板回收率为:(82.67±5.58)%;过滤前后pH值、血小板低渗休克反应值的差异无统计学意义,其余指标的差异有统计学意义。结论将浓缩血小板进行汇集、过滤白细胞,可有效去除白细胞,质量指标符合相关标准要求,且过滤前后血小板功能无显著变化,值得临床推广应用。

关键词: 浓缩血小板, 汇集, 滤除白细胞, 质量指标

Abstract: ObjectiveTo evaluate the quality of pooled platelet and leukocyte depletion,for supplying quality assurance for clinical transfusion. MethodsPlatelet concentrates (PCs) was prepared from 400 ml fresh whole blood by platelet rich plasma ( PRP) method. 12 units of ABO-matched PCs were pooled. and filtered with the domestic filters. The indicators of PCs were tested before and after filtration. ResultsThe number of platelet(Plt), red blood cell(RBC), leukocyte(WBC), pH and hypotonic shock response( HSR) before and after filtration were(3.20±0.37)x 1011 VS(2.67±0.44)x 1011,(4.39±1.97)x 109 VS(3.95±1.77)x 109,(2.06±2.02)x 108 VS(0.02±0.03)x 108, 6.78±1.18 VS 6.98±1.06,(68.53±9.18)% VS(70.96±7.81)%. The platelet recovery was(82.67±5.58)%. There were significant difference(P < 0. 05) among these indicators, such as PH,RBC and WBC. ConclusionPooling and filtering platelet concentrate is worthy of clinical use due to its standard quality and well-preserved function after filtration.

Key words: Platelet, concentrate, Pool, Filtering, out, eukodepletion, Quality, indicators

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