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临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (6): 630-635.DOI: 10.3969/j.issn.1671-2587.2017.06.030

• 仪器评价 • 上一篇    下一篇

应用Sysmex XT-4000i体液模式进行脑脊液细胞计数的性能评价*

耿丽丽, 刘义庆, 王泽筠, 沈亚娟, 亓琳, 赵万辉, 张炳昌   

  1. 250021 济南,山东大学附属省立医院临床医学检验部
  • 收稿日期:2017-08-02 出版日期:2017-12-20 发布日期:2017-12-13
  • 通讯作者: 刘义庆,男,博士,主治医师,主要从事临床检验工作,（ E-mail）yqliu1979@163.com。
  • 作者简介:耿丽丽（1989-）,女,山东济南人,检验师,学士,主要从事临床检验工作,（ E-mail）408052812@qq.com。
  • 基金资助:
    *本课题受山东省自然科学基金（No; ZR2016HM52）; 山东省科学技术发展计划资助项目（No.2014GGH218041）; 山东省临床重点专科项目（No; 鲁卫医字[2013]26号）资助

Performance Estimation of Sysmex XT-4000i in Body Fluid Mode for Counting Blood Cells in Cerebrospinal Fluid

GENG Li-li, LIU Yi-qing, WANG Ze-jun, et al   

  1. Department of Clinical Laboratory,Shandong Provincial Hospital Affiliated to Shandong University,Shandong Jinan 250021
  • Received:2017-08-02 Online:2017-12-20 Published:2017-12-13

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摘要： 目的 评估血细胞分析仪Sysmex XT-4000i体液（body fluid,BF）模式进行脑脊液细胞计数的性能。方法 收集2015年6~12月山东省立医院住院患者脑脊液标本142份,用XT-4000i进行细胞计数和分类,并与人工显微镜（人工灌板和细胞离心染色）计数进行方法学比较。结果 XT-4000i和人工显微镜计数之间白细胞计数（y=1.17x+3.21,n=118,r2=0.98）、单个核细胞计数（y=0.81x+13.61,n=101,r2=0.88）、多个核细胞计数（y=0.82x+5.15,n=101,r2=0.88）、红细胞计数（y=1.01x+146.40,n=62,r2=0.99）均呈高度相关。XT-4000i检测的研究参数高荧光强度细胞（HF-BF）水平与显微镜下查到大细胞（吞噬细胞或恶性细胞等）具有相关性（y=0.54x+3.31,n=101,r2=0.27,P<0.001）,真阳性率是13.9%,真阴性率是96.9%。XT-4000i检测的另一研究参数嗜酸性粒细胞（EO-BF）水平与人工显微镜方法的相关性无统计学意义（P>0.05）,真阳性率是9.8%,真阴性率是80.0%。XT-4000i的白细胞、单个核细胞、多个核细胞和红细胞计数均表现出良好的精密度（CV≤30%）。白细胞计数和红细胞计数具有良好的携带污染率（carry-over≤0.3%）和线性分析（r2=0.99）。结论 XT-4000i体液模式的准确度和精密度良好,可用于日常脑脊液细胞计数。高荧光强度细胞水平对脑脊液中的大细胞具有一定的提示预测作用。

关键词: 脑脊髓液, 血细胞计数, 血细胞分析仪, Sysmex XT-4000i

Abstract: Objective To evaluate the performance of the body fluid （BF）mode on Sysmex XT-4000i for counting blood cells in cerebrospinal fluid （CSF）. Methods One hundred and forty -two CSF specimens were collected from the inpatients in Shandong Provincial Hospital from June to December,2015. Blood cells of all the specimens were counted for method comparison between Sysmex XT-4000i and manual microscope （counting chamber and stained cytospin slides）.Results Excellent correlations were found for counting white blood cells （y=1.17x+3.21,n=118,r2=0.98）,mononuclear cells （y=0.81x+13.61,n=101,r2=0.88）,polynuclear cells （y=0.82x+5.15,n=101,r2=0.88）and red blood cells （y = 1.01x+146.40,n = 62,r2= 0.99）. Good agreement was found between levels of high fluorescent （HF）cells detected by XT-4000i and large cells （macrophages or malignant cells）found under the microscope （y = 0.54x+3.31,n= 101,r2= 0.27,P <0.000 1）. The true positive rate of HF cells was 13.9% and their true negative rate was 96.9%. However,no correlation was found between eosnophils detected by XT-4000i and under the microscope （P >0.05）. The true positive rate of eosnophils was 9.8% and their true negative rate was 80.0%. Good precision （CV≤30%）was got in white blood cells,mononuclear cells,polynuclear cells and red blood cells counted by XT-4000i. Both carry-over （≤0.3%）and linearity （r2=0.99）were also excellent for white blood cells and red blood cells. Conclusion The BF mode on XT-4000i may be routinely used for CSF cells count because of its excellent accuracy and precision. HF cells may have some predictive value in large cells in CSF.

Key words: Cerebrospinal fluid, Blood cell count, Hematology analyzer, Sysmex XT-4000i

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