• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2020, Vol. 22 ›› Issue (3): 322-325.DOI: 10.3969/j.issn.1671-2587.2020.03.025

• 临床检验 • 上一篇    下一篇

新型冠状病毒肺炎患者IgM抗体与血细胞计数及C反应蛋白的相关性研究*

黄琼, 徐云云, 刘海燕, 李宗光, 吴萍, 胡安群   

  1. 246003 安庆,安徽医科大学附属安庆市立医院检验科
  • 收稿日期:2020-03-10 出版日期:2020-06-20 发布日期:2020-06-13
  • 通讯作者: 胡安群,男,主任技师,主要从事临床免疫学研究,(E-mail)haq0828@hotmail.com。
  • 作者简介:黄琼(1989-),女,硕士,主管检验师,主要从事临床免疫学研究,(E-mail)383695676@qq.com。
  • 基金资助:
    *本课题受安庆市新型冠状病毒感染疫情应急科技计划项目(No.2020Z1004)资助

Study on the Correlation between IgM Antibody and Bloodcell Count and C-reactive Protein in Patients with COVID-19 Convalescent Plasma

HUANG Qiong, XU Yun-yun, LIU Hai-yan, et al   

  1. Department of Clinical Laboratory,Anqing City Affiliated Hospital of Anhui Medical University, Anhui 246004
  • Received:2020-03-10 Online:2020-06-20 Published:2020-06-13

摘要: 目的 探讨白细胞计数、C反应蛋白及新型冠状病毒(SARS-CoV-2)特异性IgM抗体在新型冠状病毒感染的肺炎(COVID-19)患者临床诊断中的应用价值。方法 对59例COVID-19确诊患者疾病早期的外周血白细胞、淋巴细胞及C反应蛋白进行检测分析,并采用胶体金免疫层析技术检测不同病程患者血清中SARS-CoV-2特异性IgM抗体的水平。结果 ①59例确诊患者中,白细胞计数正常或降低者为57例(96.61%),淋巴细胞计数降低为20例(33.9%),C反应蛋白升高者为41例(69.49%)。COVID-19患者与健康人群相比,外周血白细胞、淋巴细胞及C反应蛋白的差异有统计学意义(P< 0.05);②59例确诊患者中,52例(88.14%)患者血清SARS-CoV-2特异性IgM抗体检测为阳性,健康体检者IgM抗体均为阴性。COVID-19患者在病程第(0~6)d、(7~10)d、(11~15)d、(16~20)d血清SARS-CoV-2特异性IgM抗体阳性率分别为25%、81.82%、90%、100% 。③IgM阳性组与IgM阴性组比较,外周血白细胞、淋巴细胞及C反应蛋白水平的差异均无统计学意义(P>0.05)。结论 ①机体感染SARS-CoV-2后,外周血白细胞计数一般正常或减低,部分患者出现淋巴细胞减少,常伴有C反应蛋白升高。动态观察白细胞、淋巴细胞和C反应蛋白的变化,对于COVID-19的临床诊断和病情观察具有重要意义。②胶体金免疫层析技术检测患者血清中的SARS-CoV-2特异性IgM抗体可用于SARS-CoV-2感染患者的辅助诊断。

关键词: 新型冠状病毒, 新型冠状病毒肺炎, 血细胞计数, C反应蛋白, IgM抗体

Abstract: Objective To investigate the clinical value of blood cell count, C-reactive protein and novel coronavirus (SARS-CoV-2) specific IgM antibody in the diagnosis of COVID-19. Methods The peripheral blood leukocyte, lymphocyte counts and C-reactive protein in 59 patients diagnosed with COVID-19 were detected and analyzed,and the level of SARS-CoV-2 specific IgM antibody in serum of patients with different course of disease were detected by colloidal gold immunochromatography. Results ①Among the 59 patients, 57 (96.61%) had normal or decreased leukocyte count,20 (33.9%) had decreased lymphocyte count and 41 (69.49%) had increased C-reactive protein.Compared with the healthy people, there were significant differences in the counts of leukocytes, lymphocytes and C-reactive protein in the patients with COVID-19(P< 0.05). ②Ofthe 59 confirmed patients, 52 (88.14%) had positive SARS-CoV-2 specific IgM antibody in their serum, while the healthy subjects had negative IgM antibody. In patients with COVID-19, the positive rates of serum SARS-CoV-2 specific IgM antibodies were 25%, 81.82%, 90%, and 100% during the course of 0-6 d, 7-10 d, 11-15 d, 16-20 d, respectively. ③There was no significant difference in the levels of peripheral blood leukocytes, lymphocytes and C-reactive protein between the IgM positive group and the IgM negative group (P>0.05). Conclusion ①After infection with SARS-CoV-2,the leukocyte count in peripheral blood was generally normal or decreased,and some patients presented lymphocytopenia, often accompanied by elevated C-reactive protein.Dynamic observation of the changes of leukocytes, lymphocytes and C-reactive protein is of great significance for the clinical diagnosis and disease observation of COVID-19.②The detection of SARS-CoV-2 specific IgM antibodies in the serum of patients by colloidal gold immunochromatography can be used for auxiliary diagnosis of patients with SARS-CoV-2 infection.

Key words: SARS-CoV-2, COVID-19, Bloodcount, C-reactive protein, IgM antibody

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