Abstract: Objective To analyze the results of serum markers of HBsAg，anti-HCV，anti-HIV，and anti-TP in patients of preoperation and blood transfusion. in order to avoid medical disputes，and to provide reference for control of hospital infection. MethodsThe clinical data of 15 959 patients before operation or blood transfusion from January 2013 to June 2014 were retrospectively analysized. All detections were performed by chemiluminescence. Result 1 630 of 15 959 patients suffered from HBV infections，showing 10.21% of positive rate of HBsAG，which is the highest in the four indicators. The positive rate of HBV and HCV infection in digestive medicine were 34.59% and 8.72%. Overlapping infections were mostly observed between HBV and TP with 43.37%. Anti- HIV was positive in7 patients，1 case was co-infected with HBV，and 2 cases with Treponema pallidum （TP）. Three infectious indicators of patients varied inh genders （P<0.05）except for anti-HCV. Conclusion The detection of the four serological markers of infectious dideases in patients of pre-surgery or –blood transfusion may help to discover infections and provide evidence for the potential medical disputes，reduce and prevent the occurrence of hospital infections and occupational exposure of medical staff.

Key words: Blood transfusion , Infectious disease, Occurrence exposure , Nosocomial infection