Abstract: Objective To study the immune function and inflammatory factors after the blood transfusions following leukocyte filtration in the patients with severe trauma. Methods One hundred and forty-seven patients were randomLy divided into three groups,49 patients of the first group received transfusions with white blood cells removed and 49 patients of second group were given conventional plasma products with white blood cells. The patients in the third group untreated with transfusion were taken as control. Concentrations of CD3+,CD4+,CD8+,CD4+/CD8+,TNF-α,and IL-6 were determined in all of the subjects investigated before and after transfusion on 1d and 7d . Results No difference was noted of CD3+,CD4+,CD8+,and CD4+／CD8+ in those who received WBC-removed blood transfusion（P>0.05）on day 7 while the indicators obviously decreased in those with whole blood transfusions（P<0.05）. All the patients with transfusion showed a significant increase in serum TNF-αand IL-6 （P<0.01）on 1d and gradually decreased on 7d after transfusion（P<0.01）. The level of TNF-αand IL-6 was elevated in the patients with WBC- removed transfusion on 7d after transfusion（P<0.01）.The patients of second group presented an increased level of TNF-αafter transfusion（P<0.05）but no change was seen for IL-6（P>0.05）. Conclusion Leukocytes-free transfusion may delay the decrease of serum TNF-αand IL-6,which is helpful for rehabilitation of the patients with blood transfusion.

Key words: Blood transfusion, Leukocyte filtration, Immune indicators, Inflammatory factors