Abstract: Objective To compare the effects of 0.2 mol/L dithiothreitol（DTT）, 0.2 mol/L 2-mercaptoethanol （2-ME） and 1% bromelin on the removal of CD38 antigen on the surface of red blood cells（RBCs）. Methods 2%~5% RBCs suspension was mixed with 0.2 mol/L DTT, 0.2 mol/L 2-ME, and 1% bromelin in a volume ratio of 1∶4 respectively, and then incubated at 37℃ for 30 minutes. Untreated RBCs were used as positive control. Indirect anti-human globulin test（IAT）was performed with the RBCs suspension and the plasma of patients with Daratumumab（Anti-CD38）. Results The results showed that no agglutination was found in DTT and 2-ME treated RBCs, that weak agglutination was observed under microscope in bromelin treated RBCs, and that 1+ agglutination was in positive control. It was found that hemolysis occurred in all treated RBCs during the IAT procedure, and that bromelin treated RBCs were shown most serious hemolysis. Conclusion All three reagents can remove CD38 antigen on the surface of RBCs, and the removal efficiency of 0.2 mol/L DTT and 0.2 mol/L 2-ME is better than that of 1% bromelin.

Key words: CD38 antigen, DTT, 2-ME, Bromelin