Abstract: Objective In this paper, we analyzed the changes of related indexes of platelet and white blood cell in the blood routine of male apheresis platelet donors with different total times of platelet donation, then evaluated the effect of donation times on the peripheral hematology related indicators. Methods 448 voluntary male blood donors were enrolled, who had successfully donated platelet in our blood center from April 23 2020 to April 23 2021(the total number of blood donations were one time, ten times, multiple times of ten, and more than one hundred times respectively, among which, the apheresis platelet donors with a total number of donations more than ten times donate platelet at least 8 times a year,) the changes of the following indicators in the peripheral blood of the donors before the last platelet donation were compareed, including PLT, PDW, MPV, P-LCR, PCT, WBC, NEUT#, LYMPH#, MONO#, EO# and BASO#. Results Compared with first-time male apheresis platelet donors, there were significant differences in WBC, PLT, PCT, NEUT# and LYMPH# among apheresis platelet donors with more than hundreds total blood donations (P<0.01). Although the WBC, PCT and NEUT were significant between the two groups#, the median of these three indicators were still within the range of normal reference values, and PLT was within the normal range required for the blood testing (≥150×10⁹/L and <450×10⁹/L). The incidence of LYMPH # lower than the normal reference value was 86.21% in male pheresis platelet donors who donated blood more than 100 times, while that was 6.37% in those who donated platelet for the first time, and there was a significant difference between the above two groups. The more blood donations, the lower the overall level of LYMPH # of platelet donors. Among the platelet donors who donated blood 20 and 30 times, the proportion of LYMPH # lower than the normal reference value accounted for 46.38%, and with the increase of platelet donation times, the proportion of that gradually increased. Conclusion The results indicated multiple pheresis platelet donation would lead to platelet donors' LYMPH # levels lower than the normal reference value, and blood collection and supply institutions should pay attention to this phenomenon in the recruitment of apheresis platelet dononrs.

Key words: Multiple platelet, Donation male, Apheresis platelet donors, Blood routine, Lymphocyte