Abstract: Objective To investigate the clinical value of anti-HCV-IgG antibody and serum HCV-RNA in the diagnosis of hepatitis C. Methods The serum samples of 114 patients with hepatitis C were collected from October 2014 to October 2016,the anti-HCV-RNA antibody was detected by enzyme-linked immunosorbent assay （ELISA）,and the HCV-RNA load was detected by fluorescence quantitative polymerase chain reaction （PCR）.Statistics of all specimens of anti-HCV-RNA antibody,HCV-RNA data. Results The positive rate of anti-HCV-IgG was 25.44% （29/114）by ELISA. The positive rate of HCV-RNA was 27.19% （31/114）by fluorescence quantitative PCR. anti-HCV-IgG positive specimens of 26 cases were detected from 31 cases of HCV-RNA positive specimens,the coincidence rate of 83.87% （26/31）. There was no significant difference in detection positive rate between anti-HCV-IgG antibody by ELISA and HCV-RNA by fluorescence quantitative PCR （P> 0.05）. With the increase of HCV-RNA viral load,the detection rate of anti-HCV-IgG positive antibody was significantly increased. There was no significant difference in the detection rate of anti-HCV-IgG antibody positive in the adjacent region of different HCV-RNA viral load （P> 0.05）.Conclusion ELISA to detect anti-HCV-RNA antibody and fluorescence quantitative PCR detection of HCV-RNA in the diagnosis of hepatitis C have a certain clinical value,serum HCV-RNA viral load is an important basis for judging the infection of hepatitis C virus,which can But a single anti-HCV-RNA antibody,HCV-RNA detection is still limited,prone to misdiagnosis phenomenon,the combination of the two can significantly improve the detection rate of hepatitis C virus infection,Do early diagnosis,early treatment,to provide value for improving clinical efficacy.

Key words: Anti-HCV-IgG antibody, HCV-RNA, Hepatitis C, Diagnosis, Clinical value