Abstract: Objective To investigate the effect of SENEX on the proliferation and apoptosis of Acute Myeloid Leukemia (AML) cells. Methods 20 adult patients with newly diagnosed AML, 5 relapsed AML, 17 complete remission of AML, and 7 control bone marrow samples were selected. The expression level of SENEX was detected by qPCR, apoptosis was detected by flow cytometry, and cell proliferation was detected by CCK8. Results (1) The expression level of SENEX increased in newly diagnosed and relapsed AML, and was related to the disease status; (2) After sliencing with SENEX, the cell proliferation efficiency of NB4 cells decreased and the proportion of apoptosis increased; (3) IL-35 can attenuate the apoptotic proportion of NB4 cell and the upward trend of SENEX gene induced by cytarabine . Conclusions enex might participate in the occurrence and development of AML by directly promoting the proliferation of AML cells and inhibiting the apoptosis of AML cells.

Key words: Leukemia, Acute myeloid leukemia, SENEX