Abstract: Objective To understand and confirm the new human leukocyte antigen (HLA) allele and analyze its nucleotide sequence. Methods Using the direct sequencing method of polymerase chain reaction (PCR-SBT), we analyzed 7 500 hematopoietic stem cell donators with HLA-DRB1 high-resolution classification in Yueyang area and found one example appeared close to HLA-DRB1*09:01:02 sequence of new allele. We made confirmation with the sequencing technique SSP sequencing primers which used for DRB1*09 site specificity and verified the its differences from DRB1* 09:01:02 sequence. Results The gene in exon 2 compared with DRB1*09:01:02 276 C>A, its mutations can lead to codon AGC>AGA, 63 S(ser)>R(arginine), has been submitted to GenBank, the gene sequence Numbers for 1523488. Conclusion The allele was obtained by the nucleotide sequence analysis of the new HLA-DRB1 allele, which was named as HLA-DRB1*09:18 in May22, 2013 by the World Health Organization (WHO) HLA Nomenclature Committee.

Key words: New allele, Sequencing, HLA