Abstract: ObjectiveTo detect and analyze antibodies to HLA in female donators in Xi'an,and provide experimental data for improving the security of blood transfusion. MethodsSerum specimens of 202 women donators were randomly collected. and tested for HLA-Ⅰand HLA -Ⅱ antibodies using ELISA in order to reveal the differences of HLA antibodies between blood donors of non-pregnancy and those of pregnancy. ResultsFor 202 women of donations,HLA antibody of 37 donators were positive （18.3%）. The HLA antibody positive rate in pregnant women was significantly higher（30.9%） than that in non- pregnancies,The prevalence of anti-HLA increased with the frequency of pregnancies ConclusionThe female blood donators have a high positivity of anti-HLA,indicating a clinical implication of anti-HLA screening for safe transfusion in this target population.

Key words: HLA, HLA, antibody, Blood, donor