Polymorphic Analysis of HLA-E Gene in Han Population of Jiangsu Province

doi:10.3969/j.issn.1671-2587.2017.02.023

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2017, Vol. 19 ›› Issue (2): 173-175.DOI: 10.3969/j.issn.1671-2587.2017.02.023

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Polymorphic Analysis of HLA-E Gene in Han Population of Jiangsu Province

LIN Hong, SHAO Lei

Jiangsu Province Blood Center 210042

Received:2016-12-12 Online:2017-04-20 Published:2017-04-24

Abstract

Abstract: Objective To investigate the genetic polymorphisms of HLA-E locus in Han population of Jiangsu region.Methods The genomic DNA extracted from 217 unrelated healthy blood donors were amplified by using HLA-E specific PCR primers.Results Two alleles including HLA-E* 01：01 and HLA-E* 01：03 were identified,the frequencies were 41.47% and 58.53%,respectively．The frequencies of the three HLA-E genotypes（HLA-E*01：01/01：01,01：01/01：03 and 01：03/01：03）were 14.74%,53.46% and 31.80%,respectively,and they were in accordance with Hardy-Weinberg Equilibrium.Conclusion The polymorphism of HLA-E in the Han population of Jiangsu province was highly conserved,suggesting a role of HLA-E in disease association and clinical transplantation.

Key words: Human leukocyte antigens, HLA-E locus, Genetic polymorphisms

CLC Number:

R392.11

LIN Hong, SHAO Lei. Polymorphic Analysis of HLA-E Gene in Han Population of Jiangsu Province[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2017, 19(2): 173-175.

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Polymorphic Analysis of HLA-E Gene in Han Population of Jiangsu Province

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