Abstract: Objective To sequence and identify a novel HLA allele. Methods PCR-SBT method was used for HLA typing and an anomaly allele that is associated with HLA-B locus was analyzed, and sequence-specific sequencing primer（SSSP）was used for sequencing so as to confirm the site of mutation. Results The results showed an new allele of HLA-B locus. SSSP sequencing indicated that the nucleotide sequence of the novel allele is different from the highest homology allele B * 15:01:01: 01 in the second exon at position 662 A>T, resulting in the amino acid sequence at position 221 of B *15:01:01:01, mutated from histidine（H） to leucine（L）. Conclusion This is a novel HLA-B allele and has been named HLA-B * 15:201 by WHO HLA Nomenclature Committee.

Key words: HLA A novel allele, Typing, Sequencing