Micro-column Gel Immunoassay forDetection ofAntibodies against Anti-tumor Drugs

doi:10.3969/j.issn.1671-2587.2018.01.003

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2018, Vol. 20 ›› Issue (1): 7-9.DOI: 10.3969/j.issn.1671-2587.2018.01.003

• Orginal Article • Previous Articles Next Articles

Micro-column Gel Immunoassay forDetection ofAntibodies against Anti-tumor Drugs

LIU Juan, CHEN Bing-hao, LU Jiang-Min, et al

Department of Blood Transfusion,Airforce General Hospital,PLA,Beijing 100142

Received:2017-12-11 Online:2018-02-20 Published:2018-02-28

Abstract

Abstract: Objective To detect the serumantibodies against oncotherapy agentsand provide clinical datafor safety of transfusion and chemotherapy. Methods A total of 309 serum samples were randomly selected from patients with chemotherapy and antibodies were detected. Antibodies tocapecitabine and trastuzumab were detected in 157 and 152 patients,respectively. The patient's seraweremixed with O-type erythrocytes coated with the corresponding drugs,andthe micro-column gel indirect anti-human globulin method wasperformed to detect the antibodies against the two agents. Results Among 309 serum samples,eight were found to bepositive,with ageneral positive rate of2.5%,including 3 (1.9%) of Trastuzumab and 5 (3%) of capecitabin. Conclusion Detection of the antibodies against anti-tumor chemicals would be necessary toprevent from immune hemolysis and other adverse reactions post-transfusion.

Key words: Antibody, against cancer therapeutic agent, Micro-column gel assay, Immune hemolysis

CLC Number:

R979.1R446.6

LIU Juan, CHEN Bing-hao, LU Jiang-Min, et al. Micro-column Gel Immunoassay forDetection ofAntibodies against Anti-tumor Drugs[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2018, 20(1): 7-9.

References

1 Garratty G,Amdt P A. An update on drug-in-duced immune hemolytic anemia[J]. Immuno hematol,2007,23(3):105-119.
2 Garratty G. Immune hemolytic anemia caused by drugs[J]. Expert Opin Drug Safe,2012,11(4):635.
3 王德炳.血液免疫学[M].北京:北京大学.医学出版社,2009:244-246.
4 杨兰,邓颖,胡洪林,等. 奥沙利铂分别联合卡培他滨及5-氟尿嘧啶/亚叶酸钙治疗晚期胃癌的疗效观察[J]. 实用医院临床杂志,2015,(2):79-81.
5 Haley K M,Russell T B,Boshkov L,et al. Fatal carboplatin-induced immune hemolytic anemia in a child with a brain tumor[J]. J Blood Med,2014,5:55-58.
6 O'Shaughnessy J,Miles D,Vukelja S,et al. Superior survival with capecitabine plus docetaxel combination therapy in anthracycline-pretreated patients with advanced breast cancer: phase III trial results[J]. Am Soc Clin Oncol,2002,20(12):2812.
7 黄红艳,江泽飞,王涛,等. 卡培他滨单药或联合方案治疗晚期乳腺癌的疗效和安全性[J]. 中华肿瘤杂志,2011,33(11):850-853.
8 林志彬. 奥沙利铂联合卡培他滨(希罗达)在晚期胃癌患者中的效果观察[J]. 中外医疗,2014(35):124-125.
9 岳健,马飞,张灵小,等. 卡培他滨联合多西他赛或长春瑞滨治疗乳腺癌肝转移的临床观察及疗效分析[J]. 中国肿瘤临床,2013,(21):1304-1308.
10 刘小林,符一岚,朱秋燕,等. 曲妥珠单抗致不良反应分析[J]. 药学与临床研究,2017,25(1):63-66.
11 丁燕,吴洪斌. 曲妥珠单抗的不良反应及处理[J]. 药物不良反应杂志,2003,5(6):388-389.
12 曹盛. 微柱凝胶免疫实验检测患者血液中抗抗生素抗体[J]. 中国社区医师:医学专业,2012,14(25):256-257.
13 杨启修,朱自严. 药物包被的红细胞检测血液中抗抗生素抗体[J]. 现代免疫学,2010,(3):243-247.
14 李翠莹,黄菲,肖洁,等. 36287名患者红细胞不规则抗体特异性及其分布[J]. 中国输血杂志,2015,28(4):412-414.
15 徐兆云. 输血患者红细胞血型不规则抗体的分布规律及在临床输血中的意义[J]. 临床医学研究与实践,2017,2(23):42-43.

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Micro-column Gel Immunoassay forDetection ofAntibodies against Anti-tumor Drugs

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