Abstract: Objective The effect of novel coronavirus pneumonia on the antigenicity and antibody titer of some blood groups after 56℃ 30 min of virus inactivation was detected. Methods 20 samples with different blood types and 6 samples with different irregular antibodies were selected. Before and after the samples were heated and inactivated, the antigenicity and antibody titer of the samples were detected by microcolumn gel method. The antibody titer was determined by the agglutination intensity of 1+, and the changes of antigenicity and antibody titer of the samples before and after thermal inactivation were compared. Results The hemolysis of blood samples inactivated at 56 ℃ for 30 minutes was serious, which had no effect on the detection of ABO blood group antigen. The titer of irregular antibody decreased by 1~2 titers, the antibody with titer ≤ 4 would be missed, and the antibody with titer >4 would not be affected. Conclusion The heat inactivation of blood samples has certain influence on the antibody titer outside the ABO blood group system, but it can effectively reduce the risk of occupational exposure of operators.

Key words: Virus, inactivation, Blood, transfusion, compatibility, test, Antibody, titer