• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (5): 462-465.DOI: 10.3969/j.issn.1671-2587.2017.05.016

• 临床检验 • 上一篇    下一篇

血浆EB病毒DNA浓度检测在初诊鼻咽癌患者分期中的应用

凡任芝   

  1. 230001 安徽省医科大学附属安徽省立医院
  • 收稿日期:2017-04-02 出版日期:2017-10-20 发布日期:2017-10-26
  • 作者简介:凡任芝(1978-),男,安徽淮北人,主管技师,硕士,主要从事临床检验诊断学质量管理控制,(E-mail)798114@163.com。

Application of EBV-DNA Detection to Staging of Untreated Nasopharyngeal Carcinoma

FAN Ren-zhi   

  1. Laboratory Center of Provincial Hospital of Anhui Medical University,Hefei,230001
  • Received:2017-04-02 Online:2017-10-20 Published:2017-10-26

摘要: 目的 探讨血浆EBV DNA浓度检测在初诊鼻咽癌患者诊断分型中的应用价值。方法 按肿瘤淋巴结转移 (Tumor Node Metastasis,TNM)进行临床分期,对57例初诊鼻咽癌患者用荧光定量PCR技术检测血浆内EBV DNA浓度。结果 EBV DNA阳性率与患者的性别及年龄段均无相关性;TNM分期中Ⅲ期和Ⅳ期的EBV DNA浓度检测结果的差异有统计学意义(P<0.05),且Ⅳ期较高;随T分期的发展,各期EBV DNA阳性检出率增高,T2和T4期EBV DNA浓度检测结果的差异有统计学意义(P<0.05),且T4期较高;M和N分期患者的差异无统计学意义。结论 血浆EBV DNA浓度水平变化与鼻咽癌患者的临床分期呈正相关,可以作为一种初诊鼻咽癌患者的辅助诊断手段。

关键词: 实时荧光定量PCR, 鼻咽癌, EB病毒

Abstract: Objective To investigate the correlation between plasma EBV-DNA level and clinical stage of untreated nasopharyngeal carcinoma (UNPC). Methods Clinical stage of the UNPC patients was determined by tumor node metastasis (TNM),and the content of EBV DNA in 57 UNPC patients was detected by fluorescence quantitative PCR. Result EBV-DNA positive rate was not correlated with gender and age of patients,but plasma EBV-DNA concentration of stage Ⅲ patients was higher than stage Ⅳ patients (P<0.05). EBV-DNA positive rate of patients was increased with the development of T stage progress. The EBV-DNA concentration of stage T4 patients was higher than stage T2 patients (P<0.05). No significant difference of plasma EBV-DNA concentration between the stage M and stage N patients. Conclusion The level of plasma EBV-DNA concentration is positively correlated with clinical stages of nasopharyngeal carcinoma. Detection of plasma EBV-DNA by fluorescence quantitative PCR is a useful auxiliary method for UNPC diagnosis.

Key words: Real-time PCR, Nasopharyngeal carcinoma, Epstein-barr virus EBV

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