关键词: 核黄素光化学法, 灭活, 全血, 淋巴细胞功能

Abstract: Objective To study the effect of riboflavin photochemical method on the function of lymphocyte in whole blood treated with a certain dose.Methods In the experimental group,the riboflavin solution was added to whole blood with a final concentration of 160 μmol/mL,then whole blood was injected into illumination blood bag.The thickness of whole blood was approximate 1.3 mm and the ultraviolet dose was 18 J/cm².The control group came from the same whole blood units without riboflavin and ultraviolet radiation.Lymphocytes in whole blood were separated to detect lymphocyte proliferation activity using CCK-8 kit and calculate proliferation inhibition rate.Stimulated by Phytohemagglutinin (PHA) with a final concentration of 1 μg/mL,cytokines secreted from lymphocytes were detected using cytokines kits.Results The lymphocyte proliferation inhibition rate was (74.18±14.00)% after riboflavin photochemical treatment.Cytokine IFN-γ,IL- 8,IL-12,IL-10,IL-13 and IL-4decreased by 63%,35%,61%,42%,77% and 8% respectively;After stimulated by PHA,cytokine IFN-γ,IL-8,IL-12,IL-10,IL-13,IL-4 decreased by 72%,43%,40%,78%,82% and 2% respectively;there was no statistical difference in the secretion of IL-4 between two groups.Conclusion Riboflavin photochemical treatment at a certain dose would inhibit partly the proliferation and cytokine secretion of lymphocytes in the whole blood.

Key words: Riboflavin photochemical method, Inactivation, Whole blood, Lymphocyte function