去白悬浮红细胞4 ℃储存不同时间的质量评价

doi:10.3969/j.issn.1671-2587.2021.04.006

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (4): 435-442.DOI: 10.3969/j.issn.1671-2587.2021.04.006

去白悬浮红细胞4 ℃储存不同时间的质量评价

田雅娴, 詹林盛, 王小慧, 田兆菊

271016 山东第一医科大学（田雅娴,田兆菊）; 中国人民解放军军事科学院军事医学研究院（田雅娴,詹林盛,王小慧）

收稿日期:2021-03-13 出版日期:2021-08-20 发布日期:2021-08-18
通讯作者: 田兆菊,女,副教授,硕士生导师,主要从事免疫学相关研究,（E-mail）zhaojutian@163.com。共同通信作者：王小慧,女,副研究员,硕士生导师,主要从事输血安全与纳米生物技术研究,（E-mail）lovechina1980@163.com。
作者简介:田雅娴（1996-）,女,山东聊城人,硕士,临床检验诊断学专业,（E-mail）tianyaxian426@163. com。

Evaluation of Leukocyte-reduced Suspended Red Blood Cells Stored at 4 ℃ for Different Times

TIAN Ya-xian, ZHAN Lin-sheng, WANG Xiao-hui, et al

School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences,Taian, Shandong 271016

Received:2021-03-13 Online:2021-08-20 Published:2021-08-18

摘要/Abstract

摘要： 目的检测去白悬浮红细胞在4 ℃储存不同时间下的红细胞溶血率、细菌生长、生化指标、能量代谢、膜损伤及氧化应激等指标,评价其质量随储存时间的变化。方法 10份志愿者全血于采集当天留取50 mL,用一次性白细胞过滤器滤白,尽可能去掉血浆层,根据200 mL全血对应红细胞保存液量等比例加入红细胞保存液,置4 ℃冰箱冷藏保存至终末期。于第0、7、14、21、28、35和42天检测溶血率、ATP/2, 3-DPG、Na⁺/K⁺/GLU/LDH/pH、PS/CD47、MDA/SOD等指标,第0、14、28和42天检测细菌生长情况。结果随储存时间延长,溶血率增加,从0天到42天由0.03%增加到0.4%左右,与0天相比差异有统计学意义（P<0.001）,但均未超过国标要求。无细菌生长。上清中Na⁺和GLU浓度降低,K⁺和LDH含量增高,pH下降。ATP在储存第14天开始下降,第21天降至初始值的36.4%,储存终末期下降至13.2%,与第0天相比均具显著性差异（P<0.001）。2, 3-DPG在储存第一周内升高而后开始下降,第28天降至初始值的57.5%,42天降至初始值的44.0%。储存终末期,PS外翻率增加至0.83%,红细胞膜CD47分子平均荧光强度降低至初始值的60.8%。MDA含量无明显改变,第28天时SOD活力下降,与新鲜红细胞相比具统计学差异（P<0.001）。结论通过对4 ℃储存去白悬浮红细胞进行质量评价,因两周内生化指标、能量代谢指标及携氧能力基本维持,推荐去白悬浮红的最佳使用期限在两周内。

关键词: 去白悬浮红细胞, 储存, 质量评价

Abstract: Objective We evaluated the quality of leukocyte-reduced suspended red blood cells (SRBCs) with storage time by detecting hemolysis rate, bacterial growth, biochemical indicators and indexes of energy metabolism, membrane damage and oxidative stress at 4 ℃. Methods On the day of collection, 50 mL of whole blood was collected from 10 volunteers as test specimens. Leukoreduction filters were used for the removal of leukocytes and the plasma was removed as much as possible on the day of collection. We added erythrocyte preservation solution according to a ratio of preservative solution per 200 mL of whole blood and stored at 4℃ until the end of study. The hemolysis rate, ATP/2, 3-DPG, Na⁺/K⁺/GLU/LDH/pH, PS/CD47 and MDA/SOD were tested on day 0, 7, 14, 21, 28, 35 and 42 of storage. Bacterial growth was measured on day 0, 14, 28 and 42 of storage. Results The hemolysis rate progressively increased with storage, which increased from 0.03% to 0.4% at from day 0 to 42. There were statistically significant differences compared to day 0 (P<0.001), but none of them exceeded the national standard requirements. There was no bacterial growth. Na⁺, GLU and pH decreased with increasing K⁺ and LDH levels in supernatant. ATP began to decrease on Day 14, dropping to 36.4% on Day 21 and to 13.2% at the end. There were significant differences compared to day 0 (P<0.001). 2, 3-DPG began to decline on day 7, decreased to 57.5% on day 28 and 44.0% on day 42. At the end of storage, external exposure of membrane phosphatidylserine (PS) increased to 0.83%, and the mean fluorescence intensity of CD47 decreased to 60.8%. There was no change in MDA value during storage. Activities of SOD decreased on day 28, with statistical difference compared to Day 0 (P<0.001). Conclusion By evaluating the quality of leukocyte-reduced SRBCs stored at 4 ℃, we suggested that the optimal storage period of leukocyte-reduced SRBCs was within two weeks, because indexes of biochemical, energy metabolic and oxygen-carrying capacity were basically maintained in two weeks.

Key words: Leukocyte-reduced suspended red blood cells, Storage, Quality evaluation

中图分类号:

R457.1

田雅娴, 詹林盛, 王小慧, 田兆菊. 去白悬浮红细胞4 ℃储存不同时间的质量评价[J]. 临床输血与检验, 2021, 23(4): 435-442.

TIAN Ya-xian, ZHAN Lin-sheng, WANG Xiao-hui, et al. Evaluation of Leukocyte-reduced Suspended Red Blood Cells Stored at 4 ℃ for Different Times[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(4): 435-442.

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去白悬浮红细胞4 ℃储存不同时间的质量评价

Evaluation of Leukocyte-reduced Suspended Red Blood Cells Stored at 4 ℃ for Different Times

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